Laboratório de Biotecnologia Animal Aplicada, Universidade Federal de Uberlândia , Uberlândia, MG , Brasil.
Braz J Microbiol. 2012 Apr;43(2):535-43. doi: 10.1590/S1517-83822012000200014. Epub 2012 Jun 1.
Campylobacter coli is an important species involved in human cases of enteritis, and chickens are carriers of the pathogen mainly in developing country. The current study aimed to evaluate the transmission of C. coli and its pathogenic effects in chicken embryos. Breeder hens were inoculated intra-esophageally with C. coli isolated from chickens, and their eggs and embryos were analyzed for the presence of bacteria using real-time PCR and plate culture. The viability of embryos was verified. In parallel, SPF eggs were inoculated with C. coli in the air sac; after incubation, the embryos were submitted to the same analysis as the embryos from breeder hens. In embryos and fertile eggs from breeder hens, the bacterium was only identified by molecular methods; in the SPF eggs, however, the bacterium was detected by both techniques. The results showed no relationship between embryo mortality and positivity for C. coli in the embryos from breeder hens. However, the presence of bacteria is a cause of precocious mortality for SPF embryos. This study revealed that although the vertical transmission is a possible event, the bacteria can not grow in embryonic field samples.
大肠弯曲菌是一种重要的人类肠炎病原体，在发展中国家，鸡是该病原体的主要携带者。本研究旨在评估大肠弯曲菌在鸡胚中的传播及其致病作用。种鸡经口接种来自鸡的大肠弯曲菌，通过实时 PCR 和平板培养分析鸡蛋和胚胎中细菌的存在情况，并验证胚胎的活力。同时，用大肠弯曲菌对 SPF 种蛋进行气囊接种；孵育后，对胚胎进行与种鸡胚胎相同的分析。在种鸡的胚胎和种蛋中，仅通过分子方法鉴定出细菌；然而，在 SPF 种蛋中，两种技术都检测到了细菌。结果表明，种鸡胚胎死亡率与大肠弯曲菌阳性之间没有关系。然而，细菌的存在是 SPF 胚胎早亡的一个原因。本研究表明，虽然垂直传播是一种可能的事件，但细菌不能在胚胎样本中生长。
