Ferreira Renata C, Teles Sheila A, Dias Márcia A, Tavares Viviane R, Silva Simonne A, Gomes Selma A, Yoshida Clara F T, Martins Regina M B
Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, 74605-050 Goiânia, Goiás, Brazil.
Mem Inst Oswaldo Cruz. 2006 Sep;101(6):689-92. doi: 10.1590/s0074-02762006000600019.
Hemodialysis patients are at high risk for hepatitis B virus (HBV) infection. A survey was conducted in the hemodialysis population of the state of Goiás, Central Brazil, aiming to assess the prevalence of HBV infection, to analyse associated risk factors, and also to investigate HBV genotypes distribution. A total of 1095 patients were interviewed in 15 dialysis units. Serum samples were screened for HBV serological markers by enzyme-linked immunosorbent assay. Hepatitis B surface antigen (HBsAg) positive samples were tested for HBV DNA by polymerase chain reaction and genotyped by restriction fragment length polymorphism. Global HBV infection prevalence was 29.8% (95% CI: 27.1-32.5). Multivariate analysis of risk factors showed that male gender, length of time on hemodialysis, and blood transfusion before 1993 were associated with HBV positivity. HBV DNA was detected in 65.4% (17/26) of the HBsAg-positive samples. Thirteen of 17 HBV DNA positive samples were genotyped. Genotype D (61.5%) was predominant, followed by A (30.8%), while genotype F was detected in only one (7.7%) sample.
血液透析患者感染乙型肝炎病毒（HBV）的风险很高。在巴西中部戈亚斯州的血液透析人群中进行了一项调查，旨在评估HBV感染的患病率，分析相关危险因素，并调查HBV基因型分布。在15个透析单位共采访了1095名患者。通过酶联免疫吸附试验对血清样本进行HBV血清学标志物筛查。对乙型肝炎表面抗原（HBsAg）阳性样本进行聚合酶链反应检测HBV DNA，并通过限制性片段长度多态性进行基因分型。全球HBV感染患病率为29.8%（95%CI：27.1 - 32.5）。危险因素的多变量分析表明，男性、血液透析时间长短以及1993年前输血与HBV阳性有关。在65.4%（17/26）的HBsAg阳性样本中检测到HBV DNA。对17个HBV DNA阳性样本中的13个进行了基因分型。D基因型（61.5%）占主导地位，其次是A基因型（30.8%），而仅在一个（7.7%）样本中检测到F基因型。
