An Lihui, Hu Jianying, Zhang Zhaobin, Yang Min
College of Environmental Science, Peking University, Beijing 100871, People's Republic of China.
Anal Bioanal Chem. 2006 Dec;386(7-8):1995-2001. doi: 10.1007/s00216-006-0846-y. Epub 2006 Oct 28.
A quantitative real-time reverse transcription polymerase chain reaction (Q- RT-PCR) assay was developed for quantification of vitellogenin (Vtg) mRNA normalized to beta-actin in so-iuy mullet. Vtg mRNA in liver samples of so-iuy mullet was induced after a single injection of E2 (0.01, 0.1, 1.0 microg/g body) and a dose-response relationship was obtained. This method was applied to determine Vtg mRNA in so-iuy mullet collected from Liaodong Bay, Bohai Bay, NanDaiHe, and a control site in north China. Compared to the control, a high level of Vtg mRNA expression was detected in so-iuy mullets collected from NanDaiHe, whereas no obvious difference between Vtg mRNA expression from Liaodong Bay and Bohai Bay was found. Thus, this method is expected to be useful for further studying the potential of Vtg mRNA as a biomarker for assessing estrogenic activity in marine environments using the so-iuy mullet as a bioindicator species.
建立了一种定量实时逆转录聚合酶链反应（Q-RT-PCR）检测方法，用于定量测定以β-肌动蛋白为内参的梭鱼卵黄蛋白原（Vtg）mRNA。单次注射雌二醇（E2，0.01、0.1、1.0μg/g体重）后，梭鱼肝脏样本中的Vtg mRNA被诱导表达，并获得了剂量反应关系。该方法应用于测定从辽东湾、渤海湾、南戴河以及中国北方一个对照地点采集的梭鱼中的Vtg mRNA。与对照组相比，在从南戴河采集的梭鱼中检测到高水平的Vtg mRNA表达，而辽东湾和渤海湾的梭鱼Vtg mRNA表达之间未发现明显差异。因此，预计该方法将有助于进一步研究以梭鱼作为生物指示物种，Vtg mRNA作为评估海洋环境中雌激素活性生物标志物的潜力。
