Chaloin-Dufau C, Sun T T, Dhouailly D
Laboratoire de Biochimie et de Biologie Cellulaire de la Différenciation, Université Joseph Fourier, Grenoble, France.
Cell Differ Dev. 1990 Dec 1;32(2):97-108. doi: 10.1016/0922-3371(90)90103-4.
Sequential expression of the K3/K12 keratin pair was studied during epithelial corneal differentiation, using monoclonal antibodies coupled with electrophoretic analysis. In the chick embryo, K12 appears on day 12, while K3 is present at least from day 11. By contrast, in the rabbit embryo, the expression of K12 (at 17 days) precedes that of K3 (at 21 days). In the adult rabbit, K3 is expressed without K12 in part of the limbus. Thus, within the corneal-type keratin pair, either the acidic or the basic partner appears first, according to the developmental stage or the species.
利用单克隆抗体结合电泳分析，研究了角膜上皮分化过程中K3/K12角蛋白对的顺序表达。在鸡胚中，K12在第12天出现，而K3至少从第11天就已存在。相比之下，在兔胚中，K12（17天时）的表达先于K3（21天时）。在成年兔中，角膜缘部分区域K3表达而K12不表达。因此，在角膜型角蛋白对中，根据发育阶段或物种的不同，酸性或碱性成分会先出现。
