Chaloin-Dufau C, Pavitt I, Delorme P, Dhouailly D
Centre de Recherche sur les Macromolécules Organisées, Université Joseph Fourier, Grenoble, France.
Epithelial Cell Biol. 1993 Jul;2(3):120-5.
Keratins form the intermediate filaments in almost all epithelial cells in vertebrates. The keratins of the rabbit corneal epithelium have previously been shown to be composed of a major keratin pair, formed by an acidic keratin K12 and a basic keratin K3, together with a minor keratin pair (acidic K14 and basic K5). Using electrophoretic and immunological analyses with two monoclonal antibodies, AK12 and AE5, that are monospecific for K12 and K3 respectively, we showed that the acidic keratin K12 is highly conserved throughout all the species studied except the trout, whereas the basic keratin K3 shows interspecies variations and was unequivocally identified only in mammals. Furthermore, in dog and human cornea K3 is present in small amounts compared with K12, while in mouse it is undetectable, suggesting that, in those species, the acidic keratin K12 may interact with the basic keratin K5 to form the corneal intermediate filaments.
角蛋白构成了脊椎动物几乎所有上皮细胞中的中间丝。先前已证明，兔角膜上皮的角蛋白由一对主要角蛋白（由酸性角蛋白K12和碱性角蛋白K3组成）以及一对次要角蛋白（酸性K14和碱性K5）组成。使用分别对K12和K3具有单特异性的两种单克隆抗体AK12和AE5进行电泳和免疫分析，我们发现除鳟鱼外，在所有研究的物种中酸性角蛋白K12都高度保守，而碱性角蛋白K3则存在种间差异，并且仅在哺乳动物中被明确鉴定。此外，在狗和人角膜中，与K12相比，K3的含量较少，而在小鼠中则无法检测到，这表明在这些物种中，酸性角蛋白K12可能与碱性角蛋白K5相互作用形成角膜中间丝。
