Zhu Jin-xiao, Shan Li-ying, Li Li-hua, Huang Zhao-hui, Liu A-gui, Yao Yuan
Department of Stomatology, Wuxi No. 4 Hospital Affiliated to Suzhou University, Wuxi Jiangsu 214062, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Aug;41(8):494-7.
To investigate the expression of the T cell-specific transcription factors T-bet/GATA-3 in recurrent aphthous ulcerations (RAU).
Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation from RAU patients and healthy controls. Expressions of T-bet and GATA-3 mRNA and protein in the PBMCs were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot respectively. The concentrations of gamma-interferon (gamma-IFN) and interleukin-4 (IL-4) in the serum were determined by enzyme-linked immunosorbent assay (ELISA).
The expressions of T-bet mRNA were 0.73 +/- 0.14 in the control group and 0.12 +/- 0.04 in the recurrent aphthous ulcerations group, the levels of T-bet protein expressions were 0.73 +/- 0.18 in the control group and 0.24 +/- 0.09 in the recurrent aphthous ulcerations group, there was a significant difference between the two groups (P < 0.01). The levels of GATA-3 mRNA expressions were 0.89 +/- 0.11 in the control group and 1.30 +/- 0.13 in the recurrent aphthous ulcerations group; GATA-3 protein expressions were 1.09 +/- 0.16 in the control group and 1.80 +/- 0.16 in the recurrent aphthous ulcerations group, there was a significant difference between the two groups (P < 0.01). In the control group, the concentration of gamma-interferon in the serum was (22.15 +/- 1.52) ng/L, which was significantly elevated compared with that in the recurrent aphthous ulcerations group (16.32 +/- 0.22) ng/L (P < 0.01), in the control group, the concentration of IL-4 in the serum was (25.58 +/- 2.59) ng/L, which was significantly lower than that of the recurrent aphthous ulcerations group (43.60 +/- 3.15) ng/L (P < 0.01). The ratio of gamma-IFN and IL-4 was positively correlated with the ratio of T-bet and GATA-3 mRNA and protein expression (r = 0.96, 0.94, P < 0.01).
Imbalance of transcription factors T-bet and GATA-3 may be one of the key factors in immune dysregulation of recurrent aphthous ulcerations.
探讨T细胞特异性转录因子T-bet/GATA-3在复发性阿弗他溃疡（RAU）中的表达情况。
采用密度梯度离心法从RAU患者和健康对照者中分离外周血单个核细胞（PBMC）。分别通过逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法检测PBMC中T-bet和GATA-3 mRNA及蛋白的表达。采用酶联免疫吸附测定（ELISA）法测定血清中γ-干扰素（γ-IFN）和白细胞介素-4（IL-4）的浓度。
对照组T-bet mRNA表达为0.73±0.14，复发性阿弗他溃疡组为0.12±0.04；对照组T-bet蛋白表达水平为0.73±0.18，复发性阿弗他溃疡组为0.24±0.09，两组间差异有统计学意义（P<0.01）。对照组GATA-3 mRNA表达水平为0.89±0.11，复发性阿弗他溃疡组为1.30±0.13；对照组GATA-3蛋白表达为1.09±0.16，复发性阿弗他溃疡组为1.80±0.16，两组间差异有统计学意义（P<0.01）。对照组血清中γ-干扰素浓度为（22.15±1.52）ng/L，明显高于复发性阿弗他溃疡组（16.32±0.22）ng/L（P<0.01）；对照组血清中IL-4浓度为（25.58±2.59）ng/L，明显低于复发性阿弗他溃疡组（43.60±3.15）ng/L（P<0.01）。γ-IFN与IL-4的比值与T-bet和GATA-3 mRNA及蛋白表达比值呈正相关（r=0.96，0.94，P<0.01）。
转录因子T-bet和GATA-3失衡可能是复发性阿弗他溃疡免疫失调的关键因素之一。
