[粉尘螨抗原刺激哮喘患者外周血单个核细胞中T-bet、GATA-3和FOXP3 mRNA的表达]

[Expression of T-bet, GATA-3 and FOXP3 mRNA in asthmatic peripheral blood mononuclear cells stimulated by Dermatophagoides farinae antigen].

作者信息

Guo Yin-shi, Xu Yi-ping, Xu Yan-hua

机构信息

Department of Respiratory, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

出版信息

Zhonghua Jie He He Hu Xi Za Zhi. 2007 Feb;30(2):112-5.

PMID:17445472

Abstract

OBJECTIVE

To study the expression of T-bet, GATA-3 and FOXP3 mRNA in asthmatic peripheral blood mononuclear cells (PBMC) before and after stimulation by Dermatophagoides farinae antigen (Df), and the relationship among the three transcription factors.

METHODS

Twenty-five patients of asthma and 15 healthy controls were included. The mRNA levels of T-bet, GATA-3, and FOXP3 in PBMC before and after Df stimulation were detected by semiquantitative reverse transcription-polymerase chair reaction (RT-PCR).

RESULTS

There was no statistical difference (t = 0.78, 0.38 respectively, all P > 0.05) in the levels of T-bet and FOXP3 mRNA between the patients (0.3 +/- 0.4, 0.42 +/- 0.24 respectively) and the healthy controls (0.4 +/- 0.3, 0.39 +/- 0.37 respectively). However, GATA-3 mRNA was statistically different (t = 2.27, P < 0.05) between the patients (1.0 +/- 0.3) and the healthy controls (0.8 +/- 0.3). After the stimulation of PBMC by Df, the levels of T-bet, GATA-3 and FOXP3 mRNA showed statistical difference (t = 2.30, 3.79, 2.08 respectively, P < 0.05, < 0.01, < 0.05 respectively) between the patients (0.33 +/- 0.39, 1.58 +/- 1.44, 0.11 +/- 0.32 respectively) and the healthy controls (0.03 +/- 0.40, 0.11 +/- 0.53, 0.43 +/- 0.66 respectively). The FOXP3 mRNA level in the asthmatics was negatively correlated with T-bet and GATA-3 mRNA (r = -0.46, P < 0.05, r = -0.62, P < 0.01 respectively).

CONCLUSIONS

There are a predominant expression of GATA-3 and T-bet, but a decreased expression of FOXP3 in Df-stimulated PBMCs from patients with asthma.

摘要

目的

研究粉尘螨抗原（Df）刺激前后哮喘患者外周血单个核细胞（PBMC）中T-bet、GATA-3和FOXP3 mRNA的表达情况以及这三种转录因子之间的关系。

方法

纳入25例哮喘患者和15名健康对照者。采用半定量逆转录-聚合酶链反应（RT-PCR）检测Df刺激前后PBMC中T-bet、GATA-3和FOXP3的mRNA水平。

结果

患者组（分别为0.3±0.4、0.42±0.24）与健康对照组（分别为0.4±0.3、0.39±0.37）的T-bet和FOXP3 mRNA水平差异无统计学意义（t分别为0.78、0.38，均P>0.05）。然而，患者组（1.0±0.3）与健康对照组（0.8±0.3）的GATA-3 mRNA水平差异有统计学意义（t=2.27，P<0.05）。Df刺激PBMC后，患者组（分别为0.33±0.39、1.58±1.44、0.11±0.32）与健康对照组（分别为0.03±0.40、0.11±0.53、0.43±0.66）的T-bet、GATA-3和FOXP3 mRNA水平差异有统计学意义（t分别为2.30、3.79、2.08，P分别<0.05、<0.01、<0.05）。哮喘患者的FOXP3 mRNA水平与T-bet和GATA-3 mRNA呈负相关（r分别为-0.46，P<0.05；r为-0.62，P<0.01）。