Ru Guo-qing, Zhao Zhong-sheng, Tang Qi-le, Xu Wen-juan
Department of Pathology, Zhejiang Provincial Hospital, Hangzhou 310014, China.
Zhonghua Wai Ke Za Zhi. 2006 Aug 1;44(15):1062-4.
To investigate mRNA expression of syndecan-1 and heparanase in gastric carcinoma, and their correlation with the growth-pattern, invasion, metastasis and prognosis of gastric carcinoma.
In situ hybridization technique was used to examine mRNA expression of syndecan-1 and heparanase in 118 specimens of gastric carcinoma.
The positive rates of syndecan-1 mRNA and heparanase mRNA were 42.4% and 55.9%, respectively. The expression of syndecan-1 mRNA and heparanase mRNA were related to tumor invasion depth (chi(2) = 32.95, P = 0.001; chi(2) = 23.19, P = 0.001), vessel invasion (chi(2) = 46.22, P = 0.001; chi(2) = 33.78, P = 0.001), lymph node (chi(2) = 28.62, P = 0.001; chi(2) = 25.43, P = 0.001) and distant metastasis (chi(2) = 63.30, P = 0.001; chi(2) = 65.76, P = 0.001), and syndecan-1 mRNA positive expression was related to tumor size (chi(2) = 6.25, P = 0.012). There was a negative relationship between Syndecan-1 mRNA and heparanase mRNA expression (r = -0.844, P = 0.001). The mean survival time of cases with low expression of syndecan-1 mRNA was significantly shorter than that of cases with high expression (r = 36.48, P = 0.001), and meanwhile, the mean survival time of heparanase mRNA positive cases was significantly shorter than that of cases with negative expression (r = 34.41, P = 0.001).
The mRNA expression of syndecan-1 and heparanase can predict the invasion and metastasis of gastric carcinoma, and can be used as markers of prognosis of gastric carcinoma.
研究syndecan-1和乙酰肝素酶在胃癌中的mRNA表达情况，以及它们与胃癌生长方式、侵袭、转移和预后的相关性。
采用原位杂交技术检测118例胃癌标本中syndecan-1和乙酰肝素酶的mRNA表达。
syndecan-1 mRNA和乙酰肝素酶mRNA的阳性率分别为42.4%和55.9%。syndecan-1 mRNA和乙酰肝素酶mRNA的表达与肿瘤浸润深度（χ² = 32.95，P = 0.001；χ² = 23.19，P = 0.001）、血管浸润（χ² = 46.22，P = 0.001；χ² = 33.78，P = 0.001）、淋巴结转移（χ² = 28.62，P = 0.001；χ² = 25.43，P = 0.001）和远处转移（χ² = 63.30，P = 0.001；χ² = 65.76，P = 0.001）相关，syndecan-1 mRNA阳性表达与肿瘤大小相关（χ² = 6.25，P = 0.012）。Syndecan-1 mRNA与乙酰肝素酶mRNA表达呈负相关（r = -0.844，P = 0.001）。syndecan-1 mRNA低表达病例的平均生存时间显著短于高表达病例（r = 36.48，P = 0.001），同时，乙酰肝素酶mRNA阳性病例的平均生存时间显著短于阴性表达病例（r = 34.41，P = 0.001）。
syndecan-1和乙酰肝素酶的mRNA表达可预测胃癌的侵袭和转移，并可作为胃癌预后的标志物。
