Vanícková Martina, Suttnar Jirí, Dyr Jan Evangelista
Department of Biochemistry, Institute of Hematology and Blood Transfusion, U Nemocnice 1, 12820 Prague, Czech Republic.
Platelets. 2006 Nov;17(7):470-6. doi: 10.1080/09537100600758875.
The biocompatibility of materials is frequently assessed by blood platelet adhesion, since platelet adhesion plays a considerable role in blood interaction with artificial surfaces. Blood platelets adhesion is an essential event in haemostatic and thrombotic processes. The aim of this study was to simultaneously compare simple biochemical assays widely used for evaluation of platelet static adhesion based on the determination of enzymatic activity of either lactate dehydrogenase (LDH) or acid phosphatase (ACP) in lysates of adhered platelets. Adhesion of platelets from platelet-rich plasma and washed platelets activated by either ADP or thrombin on surfaces covered with fibrinogen and well defined fibrin was studied. The results demonstrated that the amounts of adhered platelets estimated by the LDH method were significantly lower as compared with the amount obtained by ACP method. LDH but not ACP release from platelets during adhesion was shown to take place. It suggests that the LDH method should be used rather as an assay of platelet integrity. The ACP method is much more suitable for quantitative determination of platelet adhesion especially in the development and evaluation of haemocompatibility of new biomaterials.
材料的生物相容性通常通过血小板黏附来评估，因为血小板黏附在血液与人工表面的相互作用中起着相当重要的作用。血小板黏附是止血和血栓形成过程中的一个重要事件。本研究的目的是同时比较广泛用于评估血小板静态黏附的简单生化检测方法，这些方法基于测定黏附血小板裂解物中乳酸脱氢酶（LDH）或酸性磷酸酶（ACP）的酶活性。研究了富含血小板血浆中的血小板以及经ADP或凝血酶激活的洗涤血小板在覆盖有纤维蛋白原和明确纤维蛋白的表面上的黏附情况。结果表明，与ACP法获得的黏附血小板数量相比，LDH法估计的黏附血小板数量显著更低。研究表明，血小板在黏附过程中会发生LDH释放，但不会发生ACP释放。这表明LDH法更应被用作血小板完整性的检测方法。ACP法更适合用于定量测定血小板黏附，特别是在新型生物材料血液相容性的开发和评估中。
