Wang Ying, Wang You-Fang, Zhang Da-Bing
School of Life Sciences, East China Normal University, Shanghai 200062, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Oct;32(5):527-34.
A rice male-sterile mutant msp1-4 (MULTIPLE SPOROCYTE) with japonica cultivar '9522' background, was obtained in M(3) population treated with (60)Co gamma-ray. Results of genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive locus. To map this locus, an F(2) population was constructed from the cross between the msp1-4 (japonica) and 'LongTeFu B' (indica). This locus was mapped between the two InDel markers, WY-4 and WY-8, with physical distance of 247 kb. A deletion with 10 base pairs between 758 bp and 767 bp in MSP1 open reading frame was confirmed by sequence analysis, which led to pre-termination of MSP1 translation. Phenotype analysis of msp1-4 indicated that it was similar to the msp1 mutant. To insight the expression change of rice anther developmental genes in this mutant, semi-quantitative RT-PCR analysis was carried out. The results showed that the expression level of rice UDT1 and GAMYB were reduced in msp1-4, implying that UDT1 and GAMYB are possibly the downstream genes of MSP1 gene in rice pollen development.
以粳稻品种‘9522’为背景，通过钴60γ射线处理M(3)群体获得了一个水稻雄性不育突变体msp1-4（多孢母细胞）。遗传分析结果表明，雄性不育表型由一个单隐性位点控制。为了定位该位点，构建了一个F(2)群体，其亲本为msp1-4（粳稻）和‘龙特甫B’（籼稻）。该位点被定位在两个InDel标记WY-4和WY-8之间，物理距离为247 kb。通过序列分析证实，MSP1开放阅读框中758 bp至767 bp之间有一个10个碱基对的缺失，这导致MSP1翻译提前终止。msp1-4的表型分析表明，它与msp1突变体相似。为了深入了解该突变体中水稻花药发育基因的表达变化，进行了半定量RT-PCR分析。结果表明，水稻UDT1和GAMYB在msp1-4中的表达水平降低，这意味着UDT1和GAMYB可能是水稻花粉发育过程中MSP1基因的下游基因。
