College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China.
J Genet Genomics. 2010 Jan;37(1):47-55. doi: 10.1016/S1673-8527(09)60024-2.
Plant senescence plays diverse important roles in development and environmental responses. However, the molecular basis of plant senescence is remained largely unknown. A rice spontaneous mutant with the character of early senescence and male sterility (sms) was found in the breeding line NT10-748. In order to identify the gene SMS1 and the underlying mechanism, we preliminarily analyzed physiological and biochemical phenotypes of the mutant. The mutant contained lower chlorophyll content compared with the wild type control and was severe male sterile with lower pollen viability. Genetic analysis showed that the mutant was controlled by a single recessive gene. By the map-based cloning approach, we fine-mapped SMS1 to a 67 kb region between the markers Z3-4 and Z1-1 on chromo-some 8 using 1,074 F(2) recessive plants derived from the cross between the mutant sms1 (japonica) x Zhenshan 97 (indica), where no known gene involved in senescence or male sterility has been identified. Therefore the SMS1 gene will be a novel gene that regulates the two developmental processes. The further cloning and functional analysis of the SMS1 gene is under way.
植物衰老在发育和环境响应中发挥着多种重要作用。然而，植物衰老的分子基础在很大程度上仍然未知。在 NT10-748 品系中发现了一种具有早期衰老和雄性不育特征的水稻自发突变体(sms)。为了鉴定 SMS1 基因及其潜在机制，我们初步分析了突变体的生理生化表型。与野生型对照相比，突变体的叶绿素含量较低，花粉活力较低，雄性不育严重。遗传分析表明，该突变体由一个隐性单基因控制。通过基于图谱的克隆方法，我们使用来自突变体 sms1(japonica)x Zhenshan 97(indica)杂交的 1074 个 F2 隐性植物，将 SMS1 精细定位到染色体 8 上 Z3-4 和 Z1-1 标记之间的 67kb 区域，在该区域中没有发现与衰老或雄性不育相关的已知基因。因此，SMS1 基因将是一个调控这两个发育过程的新基因。SMS1 基因的进一步克隆和功能分析正在进行中。
