Xu Zhi-Ru, Li Yu-Hua
College of Life Sciences, Northeast Forestry University, Harbin 150040, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Oct;32(5):583-6.
'Tsuda' turnip (Brassica campestris L. ssp. rapa), in which roots anthocyanin pigmentation is light-sensitive, was used as the material. 'Tsuda' plants were held in darkness or irradiated with sun light and constant light for different time. Anthocyanins in root peel of 'Tsuda' turnip exposed to constant light were identified and quantified with a UV-visual spectrophotometer. The results demonstrated that the anthocyanins accumulation in 'Tsuda' was related with light-exposure time (Fig.1 and Table 1). Fragments of genes selected from the subtraction library of 'Tsuda' turnip involved in anthocyanin biosynthesis were used as probes. The Northern blotting results showed that the expression of PAL, CHS, F3H, DFR and ANS could be induced by irradiation with light and the expression of these genes was related with light exposure time. The expression of MYB was basically the same whether in darkness or in light (Figs.2,3).
以根中花青素色素沉着对光敏感的“津田”芜菁（Brassica campestris L. ssp. rapa）为材料。将“津田”植株置于黑暗中，或分别用太阳光和持续光照处理不同时间。用紫外可见分光光度计对经持续光照处理的“津田”芜菁根皮中的花青素进行鉴定和定量。结果表明，“津田”芜菁中花青素的积累与光照时间有关（图1和表1）。从“津田”芜菁花青素生物合成消减文库中筛选出的基因片段用作探针。Northern杂交结果表明，光照可诱导苯丙氨酸解氨酶（PAL）、查尔酮合酶（CHS）、黄烷酮3-羟化酶（F3H）、二氢黄酮醇4-还原酶（DFR）和花青素合成酶（ANS）基因的表达，且这些基因的表达与光照时间有关。MYB基因无论在黑暗还是光照条件下表达基本相同（图2、3）。
