白术内酯III在大鼠体内的药代动力学及组织分布

[Pharmacokinetics and tissue distribution of atractylenolide III in rats].

作者信息

Li Cui-Qin, He Lang-Chong, Deng Ting

机构信息

School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.

出版信息

Zhong Yao Cai. 2006 Aug;29(8):807-9.

PMID:17076240

Abstract

OBJECTIVE

[corrected] To establish an HPLC method for the analysis of pharmacokinetics and tissue distribution of atractylenolide III in rats.

METHODS

The biological samples were extracted with ether. The chromatographic conditions were as follows: Hypersil ODS column (150 mm x 4.6 mm, 5 microm) was used. The mobile phase was methnol/warter (67 : 33) with a flow rate of 1.0 ml/min under the column temperature of 25 degrees C, and the detection wavelength was set at 220 nm.

RESULTS

The recovery of the method was 85.12% (RSD = 5.57%). The linear range was 0.2 microg/ml - 18.5 microg/ml (r = 0.9996) in rat plasma. The Lowest Limit of detection was 0.10 microg/ ml (S/N > 3). The within-day and between-day precision were from 0.98% to 6.19% and 12.95% to 15.48%, respectively. After oral administration of atractylenolide III (100 mg/kg), the concentration-time profiles of atractylenonlide III fit a two compartment model. In main effect tissues, the atractylenolide III concentration was followed as in order C(lung) > C(cerebellum) > C(heart) > C(cerebrum), and that was C(spleen) > C(liver) > C(kidney) in eliminated tissues.

CONCLUSION

The method is accurate, stable and reliable, and can be used for the investigation of atractylenolide III in plasma and tissues of rats.

摘要

目的

建立高效液相色谱法用于分析大鼠体内白术内酯III的药代动力学及组织分布。

方法

生物样品用乙醚萃取。色谱条件如下：采用Hypersil ODS柱（150 mm×4.6 mm，5μm）。流动相为甲醇/水（67∶33），流速1.0 ml/min，柱温25℃，检测波长设定为220 nm。

结果

该方法的回收率为85.12%（相对标准偏差RSD = 5.57%）。大鼠血浆中的线性范围为0.2μg/ml - 18.5μg/ml（r = 0.9996）。最低检测限为0.10μg/ml（信噪比S/N > 3）。日内精密度为0.98%至6.19%，日间精密度为12.95%至15.48%。白术内酯III经口给药（100 mg/kg）后，白术内酯III的浓度 - 时间曲线符合二室模型。在主要效应组织中，白术内酯III的浓度顺序为C（肺）> C（小脑）> C（心脏）> C（大脑），在消除组织中为C（脾脏）> C（肝脏）> C（肾脏）。