Chen Yan-Ming, Chou Gui-Xin, Wang Zheng-Tao
Key Laboratory of Standardization of Chinese Medicine, Ministry of Education, Institutes of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhongguo Zhong Yao Za Zhi. 2007 Nov;32(21):2265-7.
To develop a RP-HPLC method for determination of beta-eudesmol in rhizome of Atractylodes lancea, and to provide valuble data for quality control of A. lancea.
The samples were separated on an Inertsil ODS-3 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (68:32). Flow rate was 1.0 mL x min(-1). The detection wavelength was set at 200 nm. Column temperature was 25 degrees C.
The contents of beta-eudesmol determinated was 0.833-4.466 mg x g(-1), The linear range of beta-eudesmol was 0.048-1.200 microg (r = 0.999 9), the average recovery was 99.3%, RSD was 1.4% (n = 9).
The method for quantitation of beta-eudesmol in A. lancea was accurate and reliable, which can be used to evaluate the quality of rhizome of A. lancea.
建立测定茅苍术根茎中β-桉叶醇含量的反相高效液相色谱法，为茅苍术的质量控制提供有价值的数据。
采用Inertsil ODS-3（4.6 mm×250 mm，5μm）色谱柱，以乙腈-水（68:32）为流动相进行分离。流速为1.0 mL·min⁻¹。检测波长设定为200 nm。柱温为25℃。
测定的β-桉叶醇含量为0.833 - 4.466 mg·g⁻¹，β-桉叶醇的线性范围为0.048 - 1.200μg（r = 0.999 9），平均回收率为99.3%，相对标准偏差为1.4%（n = 9）。
该茅苍术中β-桉叶醇定量方法准确可靠，可用于评价茅苍术根茎的质量。
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