Carpio E, Duarte C, Hinkula J, Broliden P A, Rosén J, Campal A, Gavilondo J, Wahren B, Jondal M
Hybridoma Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
AIDS Res Hum Retroviruses. 1991 Jan;7(1):97-101. doi: 10.1089/aid.1991.7.97.
Five mouse monoclonal antibodies were raised against a recombinant protein comprising the complete sequence of gag24 protein from HTLV-IIIB. All monoclonal antibodies recognized the native protein in enzyme-linked immunosorbant assay (ELISA) and Western blots. All monoclonal antibodies also cross-reacted with an human immunodeficiency virus type 2 (HIV-2) strain in western blots, whereas only one antibody detected HIV-2 p25 in ELISA. By using synthetic peptides, cross-reacting epitopes were mapped and three regions were defined. The conserved immunogenic sites were located in the carboxyterminal region of the protein. Inhibition experiments with human sera showed that this region is also immunogenic in humans.
针对包含来自HTLV-IIIB的gag24蛋白完整序列的重组蛋白，制备了五种小鼠单克隆抗体。所有单克隆抗体在酶联免疫吸附测定（ELISA）和蛋白质印迹法中均能识别天然蛋白。在蛋白质印迹法中，所有单克隆抗体也与2型人类免疫缺陷病毒（HIV-2）毒株发生交叉反应，而在ELISA中只有一种抗体能检测到HIV-2 p25。通过使用合成肽，对交叉反应表位进行了定位并确定了三个区域。保守的免疫原性位点位于该蛋白的羧基末端区域。用人血清进行的抑制实验表明，该区域在人体内也具有免疫原性。
