一种用于检测人免疫缺陷病毒核心抗原（p24）抗体的酶联免疫吸附抑制试验。

An ELISA-inhibition assay for antibody to human immunodeficiency virus core antigen (p24).

作者信息

Povolotsky J, Gold J W, Darminin P, Chein N, Baron P, Armstrong D

机构信息

Infectious Disease Service, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

出版信息

J Virol Methods. 1990 Oct;30(1):109-14. doi: 10.1016/0166-0934(90)90048-k.

DOI:10.1016/0166-0934(90)90048-k

PMID:2128300

Abstract

An ELISA-inhibition assay based on commercially available HIV-1 p24 antigen tests was developed for detecting p24 antibodies. The test is specific and simple. p24 antibody was detectable in all p24 antigen negative Western blot positive sera, but was detectable infrequently in antigen positive sera. Sera from patients with indeterminate HIV-1 reactions (individuals without evidence of HIV-1 infection who nevertheless had p24 antibody) were p24 antigen negative and p24 antibody negative in the ELISA-inhibition reaction.

摘要

基于市售的HIV-1 p24抗原检测开发了一种用于检测p24抗体的ELISA抑制试验。该试验特异且简单。在所有p24抗原阴性但蛋白免疫印迹阳性的血清中均可检测到p24抗体，但在抗原阳性血清中很少能检测到。HIV-1反应不确定的患者（无HIV-1感染证据但有p24抗体的个体）的血清在ELISA抑制反应中p24抗原阴性且p24抗体阴性。