Gilbert Sunny D, Mediatore Sarah J, Batey Robert T
Department of Chemistry and Biochemistry, University of Colorado at Boulder, Campus Box 215, Boulder, Colorado 80309-0215, USA.
J Am Chem Soc. 2006 Nov 8;128(44):14214-5. doi: 10.1021/ja063645t.
The purine riboswitch is a genetic regulatory element found in the 5'-untranslated regions of Gram-positive bacteria that regulates expression of the mRNA specifically in response to either guanine or adenine. We report that the adenine-responsive RNA element is also capable of specifically recognizing pyrimidine compounds bearing modifications at the 6- or 5,6-positions in a fashion similar to that of purine compounds. Using isothermal titration calorimetry and X-ray crystallography, the binding of these compounds is characterized.
嘌呤核糖开关是一种存在于革兰氏阳性菌5'非翻译区的基因调控元件，它能特异性地响应鸟嘌呤或腺嘌呤来调节mRNA的表达。我们报告称，腺嘌呤响应性RNA元件也能够以类似于嘌呤化合物的方式特异性识别在6位或5,6位带有修饰的嘧啶化合物。利用等温滴定量热法和X射线晶体学对这些化合物的结合进行了表征。
