Dewez D, Eullaffroy P, Popovic R, Juneau P
Department of Chemistry-TOXEN, University of Quebec in Montreal, Succ. Centre-Ville, Montreal, QC, Canada.
Photochem Photobiol. 2007 May-Jun;83(3):714-21. doi: 10.1562/2006-08-08-RA-999.
By using saturating flash, we investigated the change in the rapid fluorescence rise when Lemna minor leaf was exposed to different light conditions and treated with exogenous electron acceptors (methyl viologen and duroquinone) and electron donor (hydroxylamine). Investigation was carried out by using combined pulse amplitude modulated fluorometer and plant efficiency analyzer system, which were employed simultaneously to provide different light conditions and to induce rapid fluorescence rise respectively. We have shown that when leaf of L. minor was exposed to different conditions of illumination, rapid fluorescence rise was greatly influenced by the electron transport functions beyond quinone A-plastoquinone reduction. This was indicated by the change in both fluorescence yield and appearance time of the different transients. When exogenous electron donor (hydroxylamine) and acceptors (methyl viologen and duroquinone) were applied in in vivo condition, we showed that rapid fluorescence rise represented a reliable indicator of PSII-PSI electron transport state and energy dissipation process.
通过使用饱和闪光，我们研究了浮萍小叶暴露于不同光照条件下并用外源电子受体（甲基紫精和杜醌）及电子供体（羟胺）处理时快速荧光上升的变化。研究使用了组合脉冲幅度调制荧光计和植物效率分析仪系统，它们同时用于提供不同光照条件并分别诱导快速荧光上升。我们已经表明，当浮萍叶暴露于不同光照条件时，快速荧光上升受到醌A - 质体醌还原之外的电子传递功能的极大影响。这通过不同瞬态的荧光产量和出现时间的变化得以表明。当在体内条件下应用外源电子供体（羟胺）和受体（甲基紫精和杜醌）时，我们表明快速荧光上升代表了PSII - PSI电子传递状态和能量耗散过程的可靠指标。
