Fokkens W J, Holm A F, Rijntjes E, Mulder P G, Vroom T M
Department of Otorhinolaryngology, Erasmus University, Rotterdam, The Netherlands.
Int Arch Allergy Appl Immunol. 1990;93(1):66-72. doi: 10.1159/000235281.
Little is known about cellular infiltrates in nasal mucosa and the differences between these infiltrates in allergic and non-allergic patients. A reproducible and objective method making use of monoclonal antibodies for the quantification and characterization of cellular infiltrates in biopsy specimens of nasal mucosa is described. This method was used to study quantitative differences in cellular infiltrates in the epithelium and lamina propria of the nasal mucosa of patients with isolated grass pollen allergy, non-allergic patients with nasal polyps, and controls. A surprisingly wide variation was found in all groups. In all groups the T lymphocytes were much more numerous than the B lymphocytes. The number of CD8+ cells exceeded the number of CD4+ cells in the epithelium but in the lamina propria the numbers were approximately equal. Significant differences between the three groups were found with respect to the number of CD1+, IgE+, neutrophils and cytoplasmic IgG4+ cells. No significant differences were found in the numbers of CD4+, CD8+, CD14+, CD22+, HLA-DR+, IgG1-3+ cells or eosinophils. The use of biopsy in combination with monoclonal antibodies is an easy and well-tolerated method to study immunological reactions in the nasal mucosa. The results of this study indicate a possible role for a T-cell-mediated response in allergic rhinitis.
关于鼻黏膜中的细胞浸润以及变应性和非变应性患者这些浸润之间的差异，人们了解甚少。本文描述了一种利用单克隆抗体对鼻黏膜活检标本中的细胞浸润进行定量和表征的可重复且客观的方法。该方法用于研究单纯草花粉变应性患者、鼻息肉非变应性患者及对照组鼻黏膜上皮和固有层中细胞浸润的定量差异。所有组均发现了惊人的广泛差异。所有组中T淋巴细胞均比B淋巴细胞多得多。上皮中CD8 +细胞数量超过CD4 +细胞，但固有层中二者数量大致相等。三组在CD1 +、IgE +、中性粒细胞和细胞质IgG4 +细胞数量方面存在显著差异。在CD4 +、CD8 +、CD14 +、CD22 +、HLA - DR +、IgG1 - 3 +细胞或嗜酸性粒细胞数量上未发现显著差异。活检联合单克隆抗体的应用是一种研究鼻黏膜免疫反应的简便且耐受性良好的方法。本研究结果表明T细胞介导的反应在变应性鼻炎中可能发挥作用。
