Ikemoto Tetsuya, Yamaguchi Takeshi, Morine Yuji, Imura Satoru, Soejima Yuji, Fujii Masahiko, Maekawa Yoichi, Yasutomo Koji, Shimada Mitsuo
Department of Immunology and Paracytology, Institute of Health Bioscience, Graduate School of Medicine, The University of Tokushima, Kuramoto, Tokushima, Japan.
Pancreas. 2006 Nov;33(4):386-90. doi: 10.1097/01.mpa.0000240275.68279.13.
Further metastasis should be avoided in pancreatic cancer (PC) patients for effective surgical treatment. Regulatory T cells (Foxp3CD4 T cells including CD4CD25 T cells and CD4CD25 T cells) play important roles in tumor immunity. This study aimed to investigate whether regulatory T cells participate in metastasis.
Peripheral blood was withdrawn from PC patients, as well as healthy volunteer donors as controls. The peripheral blood mononuclear cells (PBMCs) were subjected to FACScan analysis after labeling with anti-CD4, anti-CD25, and anti-Foxp3 antibodies. Tumor markers, including DUPAN2 and CA19-9, surface markers, such as the CD4/CD8 ratio, and the CD57 cell population were assessed. Clinical stages were classified according to the TNM classification.
The Foxp3CD4 T-cell population among the PBMCs was significantly increased in PC patients (8.10% +/- 4.65%) compared with healthy donors (2.47 +/- 0.78%) (P < 0.001). No significant relationships existed for the tumor markers, CD4/CD8 ratio, and CD57 cells. However, a significant correlation was found between Foxp3CD4 T cells among the PBMCs and the TNM stage (P < 0.05).
Foxp3CD4 T cells are good markers for metastasis detection in PC patients and more accurate than other conventional tumor markers, especially at advanced stages of the disease.
为实现有效的手术治疗，应避免胰腺癌（PC）患者发生进一步转移。调节性T细胞（包括CD4⁺CD25⁺ T细胞和CD4⁺CD25⁻ T细胞的Foxp3⁺CD4⁺ T细胞）在肿瘤免疫中发挥重要作用。本研究旨在调查调节性T细胞是否参与转移过程。
采集PC患者以及作为对照的健康志愿者捐献者的外周血。外周血单个核细胞（PBMC）用抗CD4、抗CD25和抗Foxp3抗体标记后进行流式细胞仪分析。评估包括DUPAN2和CA19-9在内的肿瘤标志物、诸如CD4/CD8比值等表面标志物以及CD57细胞群体。根据TNM分类法对临床分期进行分类。
与健康捐献者（2.47%±0.78%）相比，PC患者PBMC中Foxp3⁺CD4⁺ T细胞群体显著增加（8.10%±4.65%）（P<0.001）。肿瘤标志物、CD4/CD8比值和CD57细胞之间不存在显著相关性。然而，PBMC中的Foxp3⁺CD4⁺ T细胞与TNM分期之间存在显著相关性（P<0.05）。
Foxp3⁺CD4⁺ T细胞是PC患者转移检测的良好标志物，比其他传统肿瘤标志物更准确，尤其是在疾病晚期。
