肾综合征出血热患者CD4+CD25+Foxp3+调节性T细胞分析
[Analysis of CD4+CD25+Foxp3+ regulatory T cells in patients of haemorrhagic fever with renal syndrome].
作者信息
Nie Shu-Tao, Li Qing, Wang Hai-Yan
机构信息
Department of Clinical Laboratory, Central Hospital of Qilu Petro-chemical Hospital Group, Weifang Medical College, Zibo 255400, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2009 Mar 31;89(12):817-9.
OBJECTIVE
To investigate the percentage and significance of CD4(+)CD25(+)Foxp3(+) regulatory T cells in the patients with haemorrhagic fever with renal syndrome (HFRS).
METHODS
Peripheral blood samples were collected from 27 HFRS patients, and 19 healthy controls to examine the proportion of the CD4(+)CD25(+)Foxp3(+) cells. RT-PCR was used to detect the Foxp3 mRNA expression in the mononuclear cells in peripheral blood. Biopsy was conducted on the HFRS patients during oliguria stage. Peripheral blood samples and morning urine samples were collected in the stages and in the very day of renal biopsy to examine the urine protein, urine creatinine (Cr), blood Cr, and creatinine clearance rate (Ccr).
RESULTS
The percentages of CD4(+)CD25(+)Foxp3(+) in the HFRS patients in the hypotension, oliguria, polyuria, and convalescent stages were 5.0% +/- 1.2%, 3.1% +/- 0.8%, 4.2% +/- 0.9%, and 5.0% +/- 1.1% respectively, all significantly lower than that of the normal control group (6.5% +/- 2.1%, all P < 0.01). The proportion of CD4(+)CD25(+)Foxp3(+)T cells in the CD4(+)T cells in the oliguria stage was significantly lower than those in other stages and that of the normal controls (P < 0.01). The percentage of CD4(+)CD25(+)Foxp3(+) in the patients with HFRS was significantly correlated with the renal injury index (r = 0.51, P < 0.01), but not significantly correlated with urine protein and renal function changes (r = 0.11 and 0.18, both P > 0.05). The expression level of Foxp3 mRNA in peripheral mononuclear cells of the HEFS patients in the oliguria stage was (503 +/- 64), significantly lower than that of the normal controls (3102 +/- 409, P < 0.01) and those of the HFRS patients in the hypotension, polyuria, and convalescent stages (1805 +/- 291, 1869 +/- 320, and 2697 +/- 420, all P < 0.01).
CONCLUSION
The proportion of CD4(+)CD25(+)Foxp3(+) regulatory T cells decreases in patients with HFRS, which may contribute to the pathological process in such disease.
目的
探讨肾综合征出血热（HFRS）患者外周血中CD4(+)CD25(+)Foxp3(+)调节性T细胞的比例及其意义。
方法
采集27例HFRS患者及19例健康对照者的外周血样本，检测CD4(+)CD25(+)Foxp3(+)细胞比例。采用逆转录-聚合酶链反应（RT-PCR）检测外周血单个核细胞中Foxp3 mRNA表达水平。对HFRS患者少尿期进行肾活检，于肾活检当天及各期采集外周血和晨尿样本，检测尿蛋白、尿肌酐（Cr）、血Cr及肌酐清除率（Ccr）。
结果
HFRS患者低血压期、少尿期、多尿期及恢复期外周血CD4(+)CD25(+)Foxp3(+)细胞比例分别为5.0%±1.2%、3.1%±0.8%、4.2%±0.9%、5.0%±1.1%，均显著低于正常对照组（6.5%±2.1%，P均<0.01）。少尿期HFRS患者CD4(+)CD25(+)Foxp3(+)T细胞在CD4(+)T细胞中的比例显著低于其他各期及正常对照组（P<0.01）。HFRS患者CD4(+)CD25(+)Foxp3(+)细胞比例与肾损伤指标显著相关（r = 0.51，P<0.01），与尿蛋白及肾功能变化无显著相关性（r = 0.11及0.18，P均>0.05）。少尿期HFRS患者外周血单个核细胞中Foxp3 mRNA表达水平为（503±64），显著低于正常对照组（3102±409，P<0.01）及HFRS患者低血压期、多尿期及恢复期（1805±291、1869±320及2697±420，P均<0.01）。
结论
HFRS患者外周血CD4(+)CD25(+)Foxp3(+)调节性T细胞比例降低，可能参与了HFRS的病理过程。
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