Hamada M, Millar J A, Simpson F O, Harris E L
Wellcome Medical Research Institute, University of Otago Medical School, Dunedin, New Zealand.
J Cardiovasc Pharmacol. 1990;16 Suppl 7:S9-11.
The growth curves of aortic smooth muscle cells (SMCs) from spontaneously hypertensive rats (SHRs) and their normotensive controls, Wistar-Kyoto (WKY) rats, and genetically hypertensive (GH) rats and their normotensive outbred controls (N) were compared. The proportion of cells in the various phases of the cell cycle was measured by flow cytometry. SHR cells showed significantly shorter doubling time than WKY rat cells, but doubling times for GH and N rats did not differ and were nearly the same as for SHRs. SHRs showed significantly greater proportion of S-phase (DNA-synthesizing) cells compared to WKY rat cells in the exponential growth phase. No significant difference was observed between GH and N rats in the proportion of cells in other phases of the cell cycle. GH and N rat cells stopped dividing in fetal calf serum (FCS)-free Dulbecco's Modified Eagle Medium (DMEM), whereas SHR and WKY rat cells continued to progress through the cell cycle in serum-free DMEM. The responses of FCS-deprived-arrested cells to the addition of FCS were similar in GH and N rats. SHRs showed a prolonged increase in S-phase cells in response to FCS compared to WKY rat cells, but maximum effects were similar. Enhanced cell proliferation in SHRs is not present in the other hypertensive strain of rats and is not explained by a simple hyperresponse to FCS.
比较了自发性高血压大鼠（SHR）及其血压正常对照Wistar-Kyoto（WKY）大鼠、遗传性高血压（GH）大鼠及其血压正常的远交对照（N）大鼠的主动脉平滑肌细胞（SMC）的生长曲线。通过流式细胞术测量细胞周期各阶段的细胞比例。SHR细胞的倍增时间明显短于WKY大鼠细胞，但GH和N大鼠的倍增时间没有差异，且与SHR的几乎相同。在指数生长期，与WKY大鼠细胞相比，SHR的S期（DNA合成期）细胞比例显著更高。在细胞周期的其他阶段，GH和N大鼠的细胞比例没有显著差异。GH和N大鼠的细胞在无胎牛血清（FCS）的杜氏改良 Eagle培养基（DMEM）中停止分裂，而SHR和WKY大鼠的细胞在无血清DMEM中继续通过细胞周期。在GH和N大鼠中，血清剥夺后停滞的细胞对添加FCS的反应相似。与WKY大鼠细胞相比，SHR对FCS的反应是S期细胞增加持续时间延长，但最大效应相似。增强的细胞增殖在其他高血压大鼠品系中不存在，也不能用对FCS的简单高反应来解释。
