Huang Hsi-Ya, Lin Heng Yi, Lin Shih-Peng
Department of Chemistry and Center for Nanotechnology, Chung Yuan Christian University, Chung-Li, Taiwan, ROC.
Electrophoresis. 2006 Dec;27(23):4674-81. doi: 10.1002/elps.200600125.
In this study, poly(styrene-divinylbenzene-vinylsulfonic acid) monolithic columns, prepared by singlestep in situ polymerization, were first used as separation columns for CEC in which vinylsulfonic acid was employed as the charge-bearing monomer. The effects of the polymerization conditions (compositions of monomer and porogenic solvent, and polymerization time) on the chromatographic property of the polystyrene-based monolithic column were examined. Furthermore, an increase in water content of the porogenic solvent markedly altered the retention behaviors of these analytes, where the monolithic column was able to be produced in a higher ratio of vinylsulfonic acid. Finally, the study demonstrated that acidic analytes could be successfully separated by the polystyrene-based monolithic column under an acidic mobile phase.
在本研究中，通过单步原位聚合制备的聚（苯乙烯 - 二乙烯基苯 - 乙烯基磺酸）整体柱首次用作毛细管电色谱的分离柱，其中乙烯基磺酸用作带电单体。考察了聚合条件（单体和致孔溶剂的组成以及聚合时间）对聚苯乙烯基整体柱色谱性能的影响。此外，致孔溶剂含水量的增加显著改变了这些分析物的保留行为，此时能够以更高比例的乙烯基磺酸制备整体柱。最后，该研究表明，在酸性流动相条件下，聚苯乙烯基整体柱能够成功分离酸性分析物。
