Huang Hsi-Ya, Liu Yen-Chen, Cheng Yi-Jie
R&D Center for Membrane Technology, Department of Chemistry and Center for Nanotechnology at CYCU, Chung Yuan Christian University, Chung-Li, Taiwan.
J Chromatogr A. 2008 May 9;1190(1-2):263-70. doi: 10.1016/j.chroma.2008.02.105. Epub 2008 Mar 7.
A new polystyrene-based monolithic stationary phase, which was prepared by single step in situ copolymerization of styrene, divinylbenzene and vinylbenzenesulfonic acid (VBSA), was developed as a separation column for capillary electrochromatography, in which VBSA was employed as the charge-bearing monomer. Polymerization time of the polystyrene-based monolith had slightly influenced the separation time of the tested analytes, but it effectively altered their separation resolutions. Furthermore, baseline separation for a wider range of acetonitrile levels of mobile phase was achieved when a monolithic column prepared by a longer polymerization time was used. This novel polystyrene-based monolithic column provided an adequate electroosmotic flow either in basic or acidic mobile phase when VBSA level was maintained at 2.6% (w/w). Finally, this proposed polystyrene-based column allowed seven tested analytes to achieve a reproducible baseline separation within 2.2 min with theoretical plate numbers higher than 164 000 plates/m.
通过苯乙烯、二乙烯基苯和乙烯基苯磺酸（VBSA）的一步原位共聚制备了一种新型的基于聚苯乙烯的整体固定相，并将其开发为毛细管电色谱的分离柱，其中VBSA用作带电荷单体。基于聚苯乙烯的整体柱的聚合时间对测试分析物的分离时间影响较小，但能有效改变其分离分辨率。此外，当使用聚合时间较长制备的整体柱时，在更宽范围的乙腈流动相水平下实现了基线分离。当VBSA水平保持在2.6%（w/w）时，这种新型的基于聚苯乙烯的整体柱在碱性或酸性流动相中均提供了足够的电渗流。最后，这种提出的基于聚苯乙烯的柱允许七种测试分析物在2.2分钟内实现可重现的基线分离，理论塔板数高于164000块/米。
