Blevins G T, Huang H S, Tangoku A, Mckay D W, Rayford P L
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock 72205.
Life Sci. 1991;48(16):1565-74. doi: 10.1016/0024-3205(91)90281-f.
This study examines the influence of ovariectomy and administration of a pharmacologic dose of estradiol on amylase release from isolated-dispersed rat pancreatic acini and cholecystokinin receptors on rat acinar cell membranes. Rats were sham ovariectomized (intact) or ovariectomized (Ovx) and 21 day timed release pellets containing either estradiol (2.5 mg) or vehicle, were implanted subcutaneously. Eighteen days later, pancreatic acini were isolated from rats by collagenase digestion and differential centrifugation. Total cellular amylase, basal and cholecystokinin octapeptide (CCK8) stimulated amylase release and CCK membrane receptors were measured. Acini isolated from estradiol treated Ovx rats had significantly greater total cellular amylase, compared to acini isolated from either intact or Ovx rats. The amplitude of both total stimulated amylase release and percent total stimulated amylase release were significantly greater for acini isolated from vehicle treated Ovx rats, than acini isolated from either intact or estradiol treated Ovx rats. The magnitude of percent total amylase release of acini isolated from estradiol treated Ovx rats was significantly lower than that of acini isolated from intact rats. Cholecystokinin receptor concentration was significantly greater on membranes prepared from vehicle treated Ovx rats, compared to membranes prepared from either intact or estradiol treated Ovx rats. These data indicate that ovariectomy is associated with increased responsiveness of pancreatic acini to CCK stimulation, while chronic estradiol treatment of ovariectomized rats is associated with increased total cellular amylase and decreased acinar cell responsiveness to CCK8. Estrogen mediated alterations in acinar cell amylase content and amylase release may play a role in estrogen related pancreatitis.
本研究考察了卵巢切除术及给予药理剂量的雌二醇对从分离分散的大鼠胰腺腺泡释放淀粉酶以及大鼠腺泡细胞膜上胆囊收缩素受体的影响。将大鼠进行假卵巢切除（完整）或卵巢切除（Ovx），并皮下植入含雌二醇（2.5毫克）或赋形剂的21天定时释放微丸。18天后，通过胶原酶消化和差速离心从大鼠分离胰腺腺泡。测量总细胞淀粉酶、基础及胆囊收缩素八肽（CCK8）刺激的淀粉酶释放以及CCK膜受体。与从完整或Ovx大鼠分离的腺泡相比，从经雌二醇处理的Ovx大鼠分离的腺泡总细胞淀粉酶显著更多。对于从经赋形剂处理的Ovx大鼠分离的腺泡，总刺激淀粉酶释放的幅度和总刺激淀粉酶释放的百分比均显著大于从完整或经雌二醇处理的Ovx大鼠分离的腺泡。从经雌二醇处理的Ovx大鼠分离的腺泡总淀粉酶释放百分比的幅度显著低于从完整大鼠分离的腺泡。与从完整或经雌二醇处理的Ovx大鼠制备的膜相比，从经赋形剂处理的Ovx大鼠制备的膜上胆囊收缩素受体浓度显著更高。这些数据表明，卵巢切除术与胰腺腺泡对CCK刺激的反应性增加有关，而对卵巢切除大鼠进行慢性雌二醇治疗与总细胞淀粉酶增加及腺泡细胞对CCK8的反应性降低有关。雌激素介导的腺泡细胞淀粉酶含量和淀粉酶释放的改变可能在雌激素相关胰腺炎中起作用。
