Fischer Geferson, Conceição Fabricio Rochedo, Leite Fábio Pereira Leivas, Dummer Luana Alves, Vargas Gilberto D'Avila, Hübner Sílvia de Oliveira, Dellagostin Odir Antônio, Paulino Niraldo, Paulino Amarílis Scremin, Vidor Telmo
Centro de Biotecnologia, Laboratório de Virologia e Imunologia, Faculdade de Veterinária, Universidade Federal de Pelotas (UFPel), CP 354, 96010-900 Pelotas, RS, Brazil.
Vaccine. 2007 Jan 26;25(7):1250-6. doi: 10.1016/j.vaccine.2006.10.005. Epub 2006 Oct 18.
Despite recent technological advances in vaccine production, most the vaccines depend on the association with adjuvant substances. This work evaluated the adjuvant capacity of an ethanol extract of green propolis associated to inactivated Suid herpesvirus type 1 (SuHV-1) vaccine preparations. Mice inoculated with SuHV-1 vaccine plus aluminum hydroxide and 5mg/dose of propolis extract presented higher levels of antibodies when compared to animals that received the same vaccine without propolis. The use of SuHV-1 vaccine with propolis extract alone did not induce significant levels of antibodies, however it was able to increase the cellular immune response, evidenced by the increase in the expression of mRNA to IFN-gamma. Besides, propolis increased the percentage of protected animals against challenge with a lethal dose of SuHV-1. The effect of green propolis extract on the humoral and cellular immune responses may be exploited for the development of effective vaccines.
尽管近期疫苗生产技术取得了进步，但大多数疫苗仍依赖与佐剂物质结合使用。本研究评估了绿色蜂胶乙醇提取物与灭活的猪1型疱疹病毒（SuHV-1）疫苗制剂联合使用时的佐剂能力。与接种不含蜂胶的相同疫苗的动物相比，接种SuHV-1疫苗加氢氧化铝和5mg/剂量蜂胶提取物的小鼠产生了更高水平的抗体。单独使用含蜂胶提取物的SuHV-1疫苗不会诱导产生显著水平的抗体，然而它能够增强细胞免疫反应，这通过IFN-γ mRNA表达的增加得到证实。此外，蜂胶提高了免受致死剂量SuHV-1攻击的动物百分比。绿色蜂胶提取物对体液和细胞免疫反应的影响可用于开发有效的疫苗。
