Sanjay K R, Kumaresan N, Akhilender Naidu K, Viswanatha S, Narasimhamurthy K, Umesh Kumar S, Vijayalakshmi G
Department of Food Microbiology, Central Food Technological Research Institute, Mysore, India.
Food Chem Toxicol. 2007 Mar;45(3):431-9. doi: 10.1016/j.fct.2006.09.004. Epub 2006 Sep 22.
Aspergillus carbonarius, an ascomycetes fungus, is known to produce pectinase in solid-state fermentation. A mutant strain of A. carbonarius UV-10046 selected for temperature tolerance over produced polygalacturonase and during growth accumulated an yellow pigment in its biomass. Since the colored fungus suggested its application for food use, the freeze-dried biomass was evaluated to assess its safety in experimental animals. Acute and sub-acute toxicity studies were conducted on both sexes of albino rats. Feeding acute doses of A. carbonarius freeze-dried biomass at 0.5-5.0g/kg body weight to adult rats did not show any symptoms of toxicity or mortality of the rats. Similarly, dietary feeding of A. carbonarius at 0.25-2.0% level (w/w) for 14 weeks did not produce any significant changes in food intake or gain in body weight of the experimental rats compared to control rats. There were no significant differences in the relative weight of vital organs, hematological parameters, macroscopic and microscopic changes in vital organs and serum enzyme levels between the experimental and control groups. The results clearly showed that acute and sub-acute oral feeding of freeze-dried whole cells of A. carbonarius mutant for 14 weeks did not produce any toxic effects in male and female rats.
黑曲霉是一种子囊菌真菌，已知其能在固态发酵中产生果胶酶。筛选出的黑曲霉UV - 10046突变株具有耐温性，过量产生多聚半乳糖醛酸酶，并且在生长过程中其生物量中积累了一种黄色色素。由于这种有色真菌表明其可用于食品，因此对冻干的生物量进行了评估，以评估其在实验动物中的安全性。对白化大鼠的雌雄两性进行了急性和亚急性毒性研究。以0.5 - 5.0克/千克体重的急性剂量给成年大鼠喂食黑曲霉冻干生物量，大鼠未出现任何中毒症状或死亡。同样，以0.25 - 2.0%（w/w）的水平给实验大鼠喂食黑曲霉14周，与对照大鼠相比，实验大鼠的食物摄入量或体重增加没有任何显著变化。实验组和对照组在重要器官的相对重量、血液学参数、重要器官的宏观和微观变化以及血清酶水平方面没有显著差异。结果清楚地表明，对雄性和雌性大鼠进行14周的黑曲霉突变株冻干全细胞急性和亚急性口服给药未产生任何毒性作用。
