大肠杆菌B染色体中msDNA操纵子的定位

Mapping of the msDNA operon in the chromosome of Escherichia coli B.

作者信息

Lim D, Maas W K

机构信息

Department of Microbiology, New York University Medical Center, New York 10016.

出版信息

Mol Microbiol. 1990 Dec;4(12):2201-4. doi: 10.1111/j.1365-2958.1990.tb00582.x.

DOI:10.1111/j.1365-2958.1990.tb00582.x

PMID:1708440

Abstract

An msDNA operon, consisting of genes for msDNA and a reverse transcriptase, is present in Escherichia coliB and absent from E. coliK12. We have found that the msDNA operon is located on a DNA fragment, longer than 15kb, that is absent from E. coliK12. Using conjugation, P1 transduction, and nucleic acid hybridization between E. coliB and E. coliK12 strains, we have located the position of the msDNA operon on the E. coliB chromosome at a site that corresponds to minute 19 on the genetic map and to position 900 on the physical map of the E. coliK12 chromosome.

摘要

一个由多顺反子msDNA和逆转录酶基因组成的操纵子存在于大肠杆菌B中，而在大肠杆菌K12中不存在。我们发现该多顺反子msDNA操纵子位于一个长度超过15kb的DNA片段上，而这个片段在大肠杆菌K12中并不存在。通过大肠杆菌B和大肠杆菌K12菌株之间的接合、P1转导及核酸杂交，我们已将多顺反子msDNA操纵子在大肠杆菌B染色体上的位置定位到了一个与遗传图谱上第19分钟、大肠杆菌K12染色体物理图谱上第900位相对应的位点。