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J Bacteriol. 1997 Dec;179(24):7865-8. doi: 10.1128/jb.179.24.7865-7868.1997.
2
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An unusual bacterial reverse transcriptase having LVDD in the YXDD box from Escherichia coli.一种来自大肠杆菌的、在YXDD框中具有LVDD的异常细菌逆转录酶。
Biochem Biophys Res Commun. 1996 Oct 14;227(2):489-93. doi: 10.1006/bbrc.1996.1534.
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Structure, function, and evolution of bacterial reverse transcriptase.细菌逆转录酶的结构、功能与进化
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Diversity of retron elements in a population of rhizobia and other gram-negative bacteria.根瘤菌和其他革兰氏阴性细菌群体中反转录子元件的多样性。
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The retron: a bacterial retroelement required for the synthesis of msDNA.反转录子：一种合成多拷贝单链DNA（msDNA）所需的细菌反转录元件。
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Multicopy single-stranded DNA isolated from a gram-negative bacterium, Myxococcus xanthus.从革兰氏阴性细菌黄粘球菌中分离出的多拷贝单链DNA。
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Extensive diversity of branched-RNA-linked multicopy single-stranded DNAs in clinical strains of Escherichia coli.大肠杆菌临床菌株中与分支RNA相连的多拷贝单链DNA的广泛多样性。
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Reverse transcriptase in a clinical strain of Escherichia coli: production of branched RNA-linked msDNA.临床分离的大肠杆菌中的逆转录酶：产生分支RNA连接的多顺反子DNA。
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DNA sequence of satellite bacteriophage P4.卫星噬菌体P4的DNA序列。
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Nucleotide sequence of the DNA packaging and capsid synthesis genes of bacteriophage P2.
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微小多拷贝单链DNA-Ec48，来自大肠杆菌的最小多拷贝单链DNA。

msDNA-Ec48, the smallest multicopy single-stranded DNA from Escherichia coli.

作者信息

Mao J R, Inouye S, Inouye M

机构信息

Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854, USA.

出版信息

J Bacteriol. 1997 Dec;179(24):7865-8. doi: 10.1128/jb.179.24.7865-7868.1997.

DOI:10.1128/jb.179.24.7865-7868.1997
PMID:9401048
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC179752/
Abstract

Previously we have reported a novel bacterial reverse transcriptase (RT) from Escherichia coli ECOR58 strains in which the YXDD box was replaced with LVDD (J.-R. Mao, S. Inouye, and M. Inouye, Biochem. Biophys. Res. Commun. 227:489-493, 1996). Here we determined the structure of the multicopy single-stranded DNA (msDNA) produced by the RT. The msDNA was found to consist of a single-stranded DNA of 48 nucleotides in length, the shortest msDNA thus far identified from natural sources. The msDNA, the RT, and the retron are designated msDNA-Ec48, RT-Ec48, and retron-Ec48, respectively. On the basis of the structure of the msr gene, the RNA molecule of msDNA-Ec48 is predicted to be composed of 119 ribonucleotides; it is the longest RNA among the known msDNAs. Analysis of the DNA sequences flanking the retron indicates that retron-Ec48 is associated with a prophage related to phages P2 and P4.

摘要

此前我们报道了一种来自大肠杆菌ECOR58菌株的新型细菌逆转录酶（RT），其中YXDD框被LVDD取代（J.-R. Mao、S. Inouye和M. Inouye，《生物化学与生物物理研究通讯》227:489 - 493，1996年）。在此我们确定了由该RT产生的多拷贝单链DNA（msDNA）的结构。发现该msDNA由一条长度为48个核苷酸的单链DNA组成，这是迄今为止从天然来源鉴定出的最短的msDNA。该msDNA、RT和反转录子分别被命名为msDNA-Ec48、RT-Ec48和反转录子-Ec48。基于msr基因的结构，预测msDNA-Ec48的RNA分子由119个核糖核苷酸组成；它是已知msDNA中最长的RNA。对反转录子侧翼DNA序列的分析表明，反转录子-Ec48与一种与噬菌体P2和P4相关的原噬菌体有关。