微小多拷贝单链DNA-Ec48，来自大肠杆菌的最小多拷贝单链DNA。
msDNA-Ec48, the smallest multicopy single-stranded DNA from Escherichia coli.
作者信息
Mao J R, Inouye S, Inouye M
机构信息
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854, USA.
出版信息
J Bacteriol. 1997 Dec;179(24):7865-8. doi: 10.1128/jb.179.24.7865-7868.1997.
Previously we have reported a novel bacterial reverse transcriptase (RT) from Escherichia coli ECOR58 strains in which the YXDD box was replaced with LVDD (J.-R. Mao, S. Inouye, and M. Inouye, Biochem. Biophys. Res. Commun. 227:489-493, 1996). Here we determined the structure of the multicopy single-stranded DNA (msDNA) produced by the RT. The msDNA was found to consist of a single-stranded DNA of 48 nucleotides in length, the shortest msDNA thus far identified from natural sources. The msDNA, the RT, and the retron are designated msDNA-Ec48, RT-Ec48, and retron-Ec48, respectively. On the basis of the structure of the msr gene, the RNA molecule of msDNA-Ec48 is predicted to be composed of 119 ribonucleotides; it is the longest RNA among the known msDNAs. Analysis of the DNA sequences flanking the retron indicates that retron-Ec48 is associated with a prophage related to phages P2 and P4.
此前我们报道了一种来自大肠杆菌ECOR58菌株的新型细菌逆转录酶（RT），其中YXDD框被LVDD取代（J.-R. Mao、S. Inouye和M. Inouye，《生物化学与生物物理研究通讯》227:489 - 493，1996年）。在此我们确定了由该RT产生的多拷贝单链DNA（msDNA）的结构。发现该msDNA由一条长度为48个核苷酸的单链DNA组成，这是迄今为止从天然来源鉴定出的最短的msDNA。该msDNA、RT和反转录子分别被命名为msDNA-Ec48、RT-Ec48和反转录子-Ec48。基于msr基因的结构，预测msDNA-Ec48的RNA分子由119个核糖核苷酸组成；它是已知msDNA中最长的RNA。对反转录子侧翼DNA序列的分析表明，反转录子-Ec48与一种与噬菌体P2和P4相关的原噬菌体有关。
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