Jablonski Elizabeth M, Mattocks M Adrian, Sokolov Eugene, Koniaris Leonidas G, Hughes Francis M, Fausto Nelson, Pierce Robert H, McKillop Iain H
Department of Biology, UNC at Charlotte, 9201 University City Blvd., Charlotte, NC, USA.
Cancer Lett. 2007 May 18;250(1):36-46. doi: 10.1016/j.canlet.2006.09.013. Epub 2006 Nov 3.
Cells undergoing apoptosis are characterized by decreased cell size due to changes in intracellular ion concentration and rapid, aquaporin (AQP)-dependent water movement out of the cell, events required for the activation of pro-apoptotic enzymes. The current study demonstrates AQP 8 and 9 expression is significantly decreased in hepatocellular carcinoma (HCC) versus normal liver. Isolation of hepatic tumor cells (H4IIE) and hepatocytes confirmed a lack of water movement across the H4IIE cell membrane via AQPs and identified an inherent resistance of H4IIE cells to apoptotic stimuli. In contrast, hepatocytes rapidly responded to osmotic challenge through AQP-dependent water movement and underwent cell death following apoptotic stimulation.
经历凋亡的细胞的特征是，由于细胞内离子浓度的变化以及水通道蛋白（AQP）依赖的水快速流出细胞，细胞大小减小，这些是激活促凋亡酶所必需的事件。当前研究表明，与正常肝脏相比，肝细胞癌（HCC）中AQP 8和9的表达显著降低。肝肿瘤细胞（H4IIE）和肝细胞的分离证实，水无法通过水通道蛋白跨H4IIE细胞膜移动，并确定H4IIE细胞对凋亡刺激具有内在抗性。相比之下，肝细胞通过AQP依赖的水移动对渗透挑战迅速做出反应，并在凋亡刺激后发生细胞死亡。
