Robertson Danielle M, Petroll W Matthew, Jester James V, Cavanagh H Dwight
Department of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390-9057, USA.
Cont Lens Anterior Eye. 2007 May;30(2):94-107. doi: 10.1016/j.clae.2006.10.001. Epub 2006 Nov 3.
Despite the development of silicone hydrogel lenses, Pseudomonas aeruginosa (PA) continues to be the leading cause of contact lens related microbial keratitis. Understanding the pathogenesis of PA-mediated corneal infection is critical to the development of new prevention and treatment strategies. Recently intracellular invasion of surface corneal epithelial cells by PA has been revisited as an important element in the infection process. This review identifies the mechanisms involved, and examines the roles of the lens, hypoxia alone, PA stain, cystic fibrosis transmembrane receptor protein (CFTR), and membrane lipid rafts in mediating intracellular invasion in both in vitro and in vivo conditions. Non-toxic blockade of raft formation in vitro or in vivo effectively abrogates PA internalization and may represent a unique, new strategy to prevent or ameliorate lens-related PA microbial keratitis.
尽管硅水凝胶镜片有所发展，但铜绿假单胞菌（PA）仍是与隐形眼镜相关的微生物性角膜炎的主要病因。了解PA介导的角膜感染发病机制对于开发新的预防和治疗策略至关重要。最近，PA对角膜表面上皮细胞的细胞内侵袭作为感染过程中的一个重要因素再次受到关注。本综述确定了其中涉及的机制，并研究了镜片、单纯缺氧、PA菌株、囊性纤维化跨膜受体蛋白（CFTR）和膜脂筏在体外和体内条件下介导细胞内侵袭中的作用。在体外或体内对脂筏形成进行无毒阻断可有效消除PA的内化，这可能代表一种独特的预防或改善与镜片相关的PA微生物性角膜炎的新策略。
