与健康对照相比，复发缓解型多发性硬化症患者外周血中CD8 + 效应记忆T细胞增加。
Increase of CD8+ T-effector memory cells in peripheral blood of patients with relapsing-remitting multiple sclerosis compared to healthy controls.
作者信息
Haegele K F, Stueckle C A, Malin J-P, Sindern E
机构信息
Department of Neurology, BG-Kliniken Bergmannsheil, Ruhr-University Bochum, Buerkle-de-la-Camp-Platz 1, 44789 Bochum, Germany.
出版信息
J Neuroimmunol. 2007 Feb;183(1-2):168-74. doi: 10.1016/j.jneuroim.2006.09.008. Epub 2006 Nov 7.
CCR7 and CD45RA expression on CD4+ and CD8+ T-cells in blood (PB) of 16 patients with multiple sclerosis (MS) and 16 healthy controls and cerebrospinal fluid (CSF) of 10 patients suffering from MS were analysed by flow cytometric measurements. T-cells were divided by their distinct homing potentials and effector-functions in three groups: naïve T-cells (CCR7+, CD45RA+), central memory T-cells (TCM) (CCR7+, CD45RA-) and effector memory T-cells (TEM) (CCR7-, CD45RA-). There was a significant increase of CD8+ TEM-cells in PB of MS patients compared to healthy controls, indicating systemic immune activation. Further we found a relative depletion of CD8+ TEM-cells in CSF of MS patients compared to matching blood samples, suggesting that these cells represent the effector arm of the immune response and infiltrate the brain tissue at the sites of inflammation.
通过流式细胞术测量分析了16例多发性硬化症（MS）患者和16名健康对照者血液（PB）中以及10例MS患者脑脊液（CSF）中CD4⁺和CD8⁺T细胞上CCR7和CD45RA的表达。根据其不同的归巢潜能和效应功能，T细胞被分为三组：初始T细胞（CCR7⁺，CD45RA⁺）、中枢记忆T细胞（TCM）（CCR7⁺，CD45RA⁻）和效应记忆T细胞（TEM）（CCR7⁻，CD45RA⁻）。与健康对照相比，MS患者PB中CD8⁺TEM细胞显著增加，表明全身免疫激活。此外，与匹配的血液样本相比，我们发现MS患者CSF中CD8⁺TEM细胞相对减少，这表明这些细胞代表免疫反应的效应臂，并在炎症部位浸润脑组织。
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