Liu Guang-zhi, Gao Xu-guang
Department of Neurology, Peking University People's Hospital, Beijing 100044, China.
Zhonghua Yi Xue Za Zhi. 2007 Oct 23;87(39):2750-2.
To investigate the phenotypes of memory T cell subsets in the patients with multiple sclerosis (MS) and further explore the mechanisms that lead to the changes of the memory T cell subsets.
Peripheral blood samples were collected from 20 MS patients, 9 with relapsing-remitting MS (RRMS) and 11 with secondary progressive multiple sclerosis (SPMS), 20 patients with cerebral infarction (disease control group), and 22 healthy persons (healthy control group). Flow cytometry and ELISA were used to detect the phenotypes of the memory T cell subsets and plasma concentration of interleukin-15 (IL-15).
The level of CD8+ TCM of the MS group was 20% +/- 11%%, significantly higher than that of the healthy control group (13% +/- 6%, P < 0.05). The level of the CD8+ terminal effector memory T cells of the MS group was 24% +/- 15%, significantly lower than that of the healthy control group (39% +/- 19%, P < 0.05). The plasma IL-15 level of the MS group was 36.01 pg/m, significantly higher than that of the healthy control group (9.53 pg/ml, P < 0.05).
The upregulation of CD8+ TCM in the MS patients may reflect a persistent chronic inflammatory response that may have been induced during early stages of the disease, while IL-15 may participate in the immunoregulatory process of promoting TCM differentiation.
研究多发性硬化症（MS）患者记忆性T细胞亚群的表型，并进一步探讨导致记忆性T细胞亚群变化的机制。
采集20例MS患者的外周血样本，其中复发缓解型MS（RRMS）患者9例，继发进展型多发性硬化症（SPMS）患者11例，20例脑梗死患者（疾病对照组），以及22名健康人（健康对照组）。采用流式细胞术和酶联免疫吸附测定法检测记忆性T细胞亚群的表型及白细胞介素-15（IL-15）的血浆浓度。
MS组CD8⁺ 中央记忆性T细胞水平为20%±11%，显著高于健康对照组（13%±6%，P<0.05）。MS组CD8⁺ 终末效应记忆性T细胞水平为24%±15%，显著低于健康对照组（39%±19%，P<0.05）。MS组血浆IL-15水平为36.01 pg/ml，显著高于健康对照组（9.53 pg/ml，P<0.05）。
MS患者CD8⁺ 中央记忆性T细胞上调可能反映了疾病早期可能诱发的持续性慢性炎症反应，而IL-15可能参与促进中央记忆性T细胞分化的免疫调节过程。
