Dutia B M, Hopkins J
Department of Veterinary Pathology, University of Edinburgh, UK.
Vet Immunol Immunopathol. 1991 Jan;27(1-3):189-94. doi: 10.1016/0165-2427(91)90099-x.
The anti-CD1 monoclonal antibodies submitted to the 1st International Workshop on Leucocyte Differentiation Antigens of Cattle, Sheep and Goats were tested for their reactivity on sheep skin, thymus and lymph node and for their reactivity with sheep efferent and afferent lymph and peripheral blood. With the exception of 20-27 they all stained that same cell populations. The antibodies precipitated molecules with a heavy chain of 46,000 apparent molecular weight and a light chain of 14,000 apparent molecular weight. VPM5 and CC14 antigens were purified by affinity chromatography. All the antibodies cross-reacted with these molecules. The results show that 20-27 recognises the same molecules as the other antibodies and suggest that 20-27 is a pan CD1 monoclonal antibody and the other monoclonal antibodies are homologues of the human CD1b molecules.
提交给第一届牛、羊白细胞分化抗原国际研讨会的抗CD1单克隆抗体，在绵羊皮肤、胸腺和淋巴结上进行了反应性测试，并测试了它们与绵羊输出和输入淋巴以及外周血的反应性。除了20 - 27之外，它们都能染色相同的细胞群体。这些抗体沉淀出重链表观分子量为46,000、轻链表观分子量为14,000的分子。通过亲和层析法纯化了VPM5和CC14抗原。所有抗体都与这些分子发生交叉反应。结果表明，20 - 27识别的分子与其他抗体相同，并表明20 - 27是一种泛CD1单克隆抗体，而其他单克隆抗体是人类CD1b分子的同源物。
