MacHugh N D, Bensaid A, Davis W C, Howard C J, Parsons K R, Jones B, Kaushal A
International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
Scand J Immunol. 1988 May;27(5):541-7. doi: 10.1111/j.1365-3083.1988.tb02381.x.
Three monoclonal antibodies (MoAb), TH97A, CC13, and CC14, define a thymic differentiation antigen in cattle. The antigen is expressed on 50-60% of bovine thymocytes, located mainly in the cortical areas, but is not expressed on peripheral blood mononuclear cells (PBMC). In cryostat sections of lymph node, the antibodies react with large dendritic-like cells in the paracortical regions. They also react with a proportion of the large 'frilly' cells in afferent lymph and with dendritic-like cells in the dermis. The antibodies apparently do not react with cells in the epidermis. Biochemical analysis of the antigen recognized by MoAb TH97A reveals two bands of 44 kDa and 12 kDa under reducing conditions. These polypeptides are distinct from bovine class I major histocompatibility complex molecules reactive with the MoAb w6/32. The tissue distribution of positive cells together with results of biochemical analyses indicate that the antigen recognized by these MoAb is the bovine analogue of the human CD1.
三种单克隆抗体（MoAb），即TH97A、CC13和CC14，可识别牛的一种胸腺分化抗原。该抗原表达于50%-60%的牛胸腺细胞上，主要位于皮质区，但在外周血单个核细胞（PBMC）上不表达。在淋巴结的冰冻切片中，这些抗体与副皮质区的大型树突状样细胞发生反应。它们还与输入淋巴管中的一部分大型“带褶边”细胞以及真皮中的树突状样细胞发生反应。这些抗体显然不与表皮细胞发生反应。对MoAb TH97A识别的抗原进行生化分析，在还原条件下显示出两条分别为44 kDa和12 kDa的条带。这些多肽不同于与MoAb w6/32反应的牛I类主要组织相容性复合体分子。阳性细胞的组织分布以及生化分析结果表明，这些MoAb识别的抗原是人类CD1的牛类似物。
