Hacker G W, Graf A H, Thurner J
Institut für Pathologische Anatomie, Landeskrankenanstalten Salzburg, Osterreich.
Verh Dtsch Ges Pathol. 1990;74:368-72.
Modern histochemical techniques allow the specific detection of tissue constituents in situ. Routinely formalin fixed, paraffin embedded tissues may present problems to the pathologist since destruction of substances can lead to false negative results. Immunogold-silver staining (IGSS) can be a way to overcome some of these problems. A highly efficient method allows silver enhancement to be carried out without the necessity of a dark box (silver acetate autometallography). To test the new modification, antisera to a variety of antigens were used on paraffin and semithin resin sections. The system was also tested for its applicability in multiple immunostaining techniques and for the in situ hybridisation of viral DNA and for demonstration of carbohydrates by lectin histochemistry. It showed a very high detection efficiency with low background staining.
现代组织化学技术可实现对组织成分的原位特异性检测。常规福尔马林固定、石蜡包埋的组织可能会给病理学家带来问题，因为物质的破坏可能导致假阴性结果。免疫金银染色（IGSS）可能是克服其中一些问题的一种方法。一种高效的方法可在无需暗箱的情况下进行银增强（醋酸银自动显影）。为测试这种新改进方法，将多种抗原的抗血清应用于石蜡和半薄树脂切片。该系统还针对其在多重免疫染色技术中的适用性、病毒DNA的原位杂交以及通过凝集素组织化学对碳水化合物的显示进行了测试。它显示出非常高的检测效率且背景染色较低。
