[用地高辛标记探针通过原位杂交检测乳头瘤病毒]
[Use of digoxigenin-labeled probes for the detection of papillomaviruses by in situ hybridization].
作者信息
Clavel C, Binninger I, Polette M, Boutterin M C, Birembaut P
机构信息
Laboratoire Pol Bouin, I.N.S.E.R.M. U. 314, Hôpital Maison-blanche, Reims.
出版信息
Ann Pathol. 1990;10(5-6):351-4.
PMID:1964785
Abstract
A technique of detection of human papillomaviruses by in situ hybridization in paraffin-embedded formalin-fixed sections of genito-anal lesions is described. The probes are labeled by incorporation of digoxigenin-labeled nucleotides. The hybrids are revealed by immunohistochemistry with an anti-digoxigenin antibody coupled with alkaline phosphatase. This technique is sensitive, cheap and may be used in routine in laboratories of pathology.
摘要
本文描述了一种在经福尔马林固定的生殖器-肛门病变石蜡包埋切片中通过原位杂交检测人乳头瘤病毒的技术。探针通过掺入地高辛标记的核苷酸进行标记。通过用与碱性磷酸酶偶联的抗地高辛抗体进行免疫组织化学来显示杂交体。该技术灵敏、成本低，可用于病理学实验室的常规检测。
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