从胚胎干细胞-体细胞杂交细胞中进行靶向染色体消除。

Targeted chromosome elimination from ES-somatic hybrid cells.

作者信息

Matsumura Hiroyuki, Tada Masako, Otsuji Tomomi, Yasuchika Kentaro, Nakatsuji Norio, Surani Azim, Tada Takashi

机构信息

Stem Cell Engineering, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

出版信息

Nat Methods. 2007 Jan;4(1):23-5. doi: 10.1038/nmeth973. Epub 2006 Nov 5.

DOI:10.1038/nmeth973

PMID:17086180

Abstract

To engineer a stem cell genome, we developed a technology for targeted elimination of chromosomes from mouse embryonic stem (ES)-somatic hybrid cells. Here we demonstrate the use of a universal chromosome elimination cassette (CEC) for elimination of a single embryonic stem cell (ESC)-derived chromosome 11 or 12, and also both copies of chromosome 6, which harbor pluripotency-associated genes including Nanog. We attribute hybrid-cell pluripotency to the expression of Nanog from the reprogrammed somatic-cell nuclei.

摘要

为了构建干细胞基因组，我们开发了一种从小鼠胚胎干细胞（ES）-体细胞杂交细胞中靶向消除染色体的技术。在此，我们展示了使用通用染色体消除盒（CEC）来消除单个源自胚胎干细胞（ESC）的11号或12号染色体，以及6号染色体的两个拷贝，6号染色体含有包括Nanog在内的多能性相关基因。我们将杂交细胞的多能性归因于重编程体细胞核中Nanog的表达。