Mall G, Klingel K, Albrecht M, Seemann M, Hofschneider P H, Kandolf R
Pathologisches Institut, Universität Heidelberg.
Verh Dtsch Ges Pathol. 1990;74:404-8.
Enteroviruses are considered as the major etiologic agents of myocarditis in humans. Recent in situ hybridization studies on endomyocardial biopsies indicate that not only in acute myocarditis (pattern of acute infection), but also in chronic dilated cardiomyopathy enterovirus RNA can be detected (pattern of persistent infection). Our experimental studies on murine coxsackievirus B3 myocarditis provided evidence that persistent infection occurs also in mice. Quantitative in-situ hybridization and immunohistochemistry as well as electron microscopic in situ hybridization experiments were performed on ACA/SnJ mice three to thirty days after infection. The pattern of acute infection (days 3-9 p.i.) is characterized by rapid progression of myocardial lesions, an increasing number of inflammatory cells and a high number of infected myocytes. Hallmarks of the persistent pattern (day 15-30 p.i.) are reduced inflammation, reduced numbers of persistently infected cells and a slow progression of myocardial lesions. Infection is primarily restricted to degenerated, atrophic myocytes and to fibroblasts.
肠道病毒被认为是人类心肌炎的主要病原体。最近对心内膜心肌活检进行的原位杂交研究表明，不仅在急性心肌炎（急性感染模式）中，而且在慢性扩张型心肌病中都能检测到肠道病毒RNA（持续感染模式）。我们对小鼠柯萨奇病毒B3心肌炎的实验研究证明，小鼠中也会发生持续感染。在感染后3至30天，对ACA/SnJ小鼠进行了定量原位杂交、免疫组织化学以及电子显微镜原位杂交实验。急性感染模式（感染后3 - 9天）的特征是心肌病变迅速进展、炎症细胞数量增加以及大量受感染的心肌细胞。持续感染模式（感染后15 - 30天）的标志是炎症减轻、持续感染细胞数量减少以及心肌病变进展缓慢。感染主要局限于退化、萎缩的心肌细胞和成纤维细胞。
