Li Pinghua, Mane Shrinivasrao P, Sioson Allan A, Robinet Cecilia Vasquez, Heath Lenwood S, Bohnert Hans J, Grene Ruth
Department of Plant Biology, University of Illinois, 1201 W Gregory Drive, Urbana, IL 61801, USA.
Plant Cell Environ. 2006 May;29(5):854-68. doi: 10.1111/j.1365-3040.2005.01465.x.
Arabidopsis thaliana (At) ecotypes Columbia-0 (Col-0), Wassilewskija (WS), Cape Verde Islands (Cvi-0) and a relative, Thellungiella halophila (Th), were exposed to 20-25% over ambient ozone [O3] in a free air concentration enrichment (FACE) experiment (http://www.soyFACE. uiuc.edu), mirroring increases expected in the near future. Col-0 and WS accelerated development and developed lesions within 10 d under increased ozone, while Cvi-0 and Th grew slowly. RNAs were used in microarray hybridizations (Col-0-based 26 000 elements, 70-mer oligonucleotides). A two-step analysis of variance (ANOVA) model, including comparison with values obtained under [O3], was used for analyses. WS showed the greatest number of changes in gene expression in response to ozone. Th showed the least changes, suggesting that its expression state at [O3] was sufficient for resistance at increased ozone. Patterns observed in ambient air controls for Cvi-0 and Col-0 were most similar, while Th showed the greatest number of differences compared with the other controls. Compared with Col-0, however, Cvi-0 showed higher levels of expression of chaperones, receptor kinase-like and photosynthesis-related genes in ambient air. Cvi-0 exhibited ozone-mediated changes in a pathway involving AtSR, a homologue of the mammalian NF kappa B family of redox-sensitive transcription factors, changes in chaperones, WRKY and C2H2 proteins and antioxidants. WS displayed ozone-mediated decreases in the expression of two AtSR/NF kappa B family members, C2-domain proteins and genes associated with cell wall growth and changes in the expression of marker genes for programmed cell death (PCD), among them RCD1, a key regulator in this pathway. Microarray data were verified by reverse transcriptase (RT)-PCR. We relate O3-response diversity across the four lines to different responses among signaling and transcriptional response networks and differences in gene expression at [O3] levels.
在一个自由空气浓度增加（FACE）实验（http://www.soyFACE.uiuc.edu）中，将拟南芥（At）的生态型哥伦比亚-0（Col-0）、瓦西列夫斯基（WS）、佛得角群岛（Cvi-0）以及一种近缘植物盐芥（Th）暴露于比环境臭氧[O₃]浓度高20 - 25%的环境中，模拟不久的将来预期的臭氧增加情况。在臭氧浓度增加的情况下，Col-0和WS在10天内加速了发育并出现了损伤，而Cvi-0和Th生长缓慢。RNA用于微阵列杂交（基于Col-0的26000个元件，70聚体寡核苷酸）。使用了两步方差分析（ANOVA）模型，包括与在[O₃]浓度下获得的值进行比较，以进行分析。WS显示出对臭氧响应中基因表达变化的数量最多。Th显示出的变化最少，这表明其在[O₃]浓度下的表达状态足以抵抗臭氧浓度增加的情况。在环境空气对照中观察到的Cvi-0和Col-0的模式最为相似，而与其他对照相比，Th显示出的差异数量最多。然而，与Col-0相比，Cvi-0在环境空气中伴侣蛋白、类受体激酶和光合作用相关基因的表达水平更高。Cvi-0在涉及AtSR（哺乳动物NFκB家族氧化还原敏感转录因子的同源物）、伴侣蛋白、WRKY和C2H2蛋白以及抗氧化剂的一条途径中表现出臭氧介导的变化。WS显示出臭氧介导的两个AtSR/NFκB家族成员、C2结构域蛋白以及与细胞壁生长相关基因的表达下降，以及程序性细胞死亡（PCD）标记基因表达的变化，其中RCD1是该途径中的关键调节因子。微阵列数据通过逆转录酶（RT）-PCR进行了验证。我们将这四个品系对臭氧的响应多样性与信号传导和转录响应网络之间的不同响应以及在[O₃]浓度水平下的基因表达差异联系起来。
