Goosen-de Roo L, de Maagd R A, Lugtenberg B J
Department of Plant Molecular Biology, Leiden University, The Netherlands.
J Bacteriol. 1991 May;173(10):3177-83. doi: 10.1128/jb.173.10.3177-3183.1991.
Three different monoclonal antibodies raised against the O antigen-containing lipopolysaccharide (LPS I) of free-living cells were used in an immunocytochemical study to follow the fate of LPS I on the outer membrane of Rhizobium leguminosarum bv. viciae 248 during the nodulation of Vicia sativa subsp. nigra. After immunogold labeling, the LPS I epitopes were detected on the outer membrane of bacteria present in infection threads throughout the nodule. Epitopes were not detectable on bacteria released from the infection thread. The data show that the LPS I epitopes present on rhizobia in infection droplets disappear shortly before or during endocytosis of the bacteria into the host plant cell cytoplasm. The abruptness of the change suggests an active degradation or modification of LPS I epitopes rather than only a repression of their synthesis.
三种针对自由生活细胞中含O抗原的脂多糖（LPS I）产生的不同单克隆抗体被用于一项免疫细胞化学研究，以追踪LPS I在豌豆根瘤菌蚕豆生物型248外膜上在黑籽野豌豆结瘤过程中的命运。免疫金标记后，在整个根瘤中感染丝内存在的细菌外膜上检测到LPS I表位。在从感染丝释放的细菌上未检测到表位。数据表明，感染滴中根瘤菌上存在的LPS I表位在细菌内吞进入宿主植物细胞质之前或期间不久消失。这种变化的突然性表明LPS I表位发生了主动降解或修饰，而不仅仅是其合成受到抑制。
