Carlson R W
J Bacteriol. 1984 Jun;158(3):1012-7. doi: 10.1128/jb.158.3.1012-1017.1984.
The lipopolysaccharides ( LPSs ) from strains of Rhizobium leguminosarum, Rhizobium trifolii, and Rhizobium phaseoli were isolated and partially characterized by mild acid hydrolysis and by polyacrylamide gel electrophoresis. Mild acid hydrolysis results in a precipitate which can be removed by centrifugation or extraction with chloroform. The supernatant contains polysaccharides which, in general, are separated into two fractions ( LPS1 and LPS2 ) by Sephadex G-50 gel filtration chromatography. The higher-molecular-weight LPS1 fractions among the various Rhizobium strains are highly variable in composition and reflect the variability reported in the intact LPSs (R. W. Carlson and R. Lee, Plant Physiol. 71:223-228, 1983; Carlson et al., Plant Physiol. 62:912-917, 1978; Zevenhuizen et al., Arch. Microbiol. 125:1-8, 1980). The LPS1 fraction of R. leguminosarum 128C53 has a higher molecular weight than all other LPS1 fractions examined. All LPS2 fractions examined are oligosaccharides with a molecular weight of ca. 600. The major sugar component of all LPS2 oligosaccharides is uronic acid. The LPS2 compositions are similar for strains of R. leguminosarum and R. trifolii, but the LPS2 from R. phaseoli was different in that it contained glucose, a sugar not found in the other LPS2 fractions or found only in trace amounts. Polyacrylamide gel electrophoretic analysis shows that each LPS contains two banding regions, a higher-molecular-weight heterogeneous region often containing many bands and a lower-molecular-weight band. The lower-molecular-weight bands of all LPSs have the same electrophoretic mobility, which is greater than that of lysozyme. The banding pattern of the heterogeneous regions varies among the different Rhizobium strains. In the case of R. leguminosarum 128C53 LPS, the heterogeneous region of a higher molecular weight than is this region from all other Rhizobium strains examined and consists of many bands separated from one another by a small and apparently constant molecular weight interval. When the heterogeneous region of R. Leguminosarum 128C53 LPS was cut from the gel and analyzed, its composition was found to be that of the intact LPS, whereas the lower-molecular-weight band contains only sugars found in the LPS2 oligosaccharide. In the case of R. leguminosarum 128C63 and R. trifolii 0403 LPSs, the heterogeneous regions are similar and consist of several band s separated by a large-molecular-weight interval with a the major band of these heterogeneous regions having the lowest molecular weight with an electrophoretic mobility near that of beta-lactoglobulin. The heterogeneous region from R. phaseoli 127K14 consists of several bands with electrophoretic mobilities near that of beta-lactoglobulin, whereas this region from R. trifolii 162S7 shows a continuous staining region, indicating a great deal of heterogeneity. The results described in this paper are discussed with regard to the reported properties of Escherichia coli and Salmonella LPSs.
从豌豆根瘤菌、三叶草根瘤菌和菜豆根瘤菌菌株中分离出脂多糖（LPSs），并通过温和酸水解和聚丙烯酰胺凝胶电泳对其进行了部分表征。温和酸水解会产生一种沉淀，可通过离心或用氯仿萃取将其除去。上清液中含有多糖，一般通过葡聚糖G - 50凝胶过滤色谱法将其分离成两个组分（LPS1和LPS2）。不同根瘤菌菌株中分子量较高的LPS1组分在组成上高度可变，反映了完整LPSs中报道的变异性（R. W. 卡尔森和R. 李，《植物生理学》71:223 - 228，1983；卡尔森等人，《植物生理学》62:912 - 917，1978；泽温胡森等人，《微生物学档案》125:1 - 8，1980）。豌豆根瘤菌128C53的LPS1组分分子量高于所检测的所有其他LPS1组分。所有检测的LPS2组分都是分子量约为600的寡糖。所有LPS2寡糖的主要糖成分是糖醛酸。豌豆根瘤菌和三叶草根瘤菌菌株的LPS2组成相似，但菜豆根瘤菌的LPS2不同，因为它含有葡萄糖，这是其他LPS2组分中未发现或仅微量存在的一种糖。聚丙烯酰胺凝胶电泳分析表明，每种LPS都包含两个条带区域，一个分子量较高的异质区域通常包含许多条带，以及一个分子量较低的条带。所有LPS的分子量较低的条带具有相同的电泳迁移率，该迁移率大于溶菌酶的迁移率。不同根瘤菌菌株的异质区域的条带模式各不相同。就豌豆根瘤菌128C53 LPS而言，其异质区域的分子量高于所检测的所有其他根瘤菌菌株的该区域，并且由许多条带组成，这些条带彼此之间以小且明显恒定的分子量间隔分开。当从凝胶中切下豌豆根瘤菌128C53 LPS的异质区域并进行分析时，发现其组成与完整LPS的组成相同，而分子量较低的条带仅包含LPS2寡糖中发现的糖。就豌豆根瘤菌128C63和三叶草根瘤菌0403 LPS而言，异质区域相似，由几个条带组成，这些条带被一个大分子量级的间隔分开，这些异质区域的主要条带具有最低的分子量，其电泳迁移率接近β - 乳球蛋白的电泳迁移率。菜豆根瘤菌127K14的异质区域由几个电泳迁移率接近β - 乳球蛋白的条带组成，而三叶草根瘤菌162S7的该区域显示出一个连续的染色区域，表明存在大量异质性。本文所描述的结果结合已报道的大肠杆菌和沙门氏菌LPSs的特性进行了讨论。
