Bal A K, Shantharam S, Verma D P
Can J Microbiol. 1980 Sep;26(9):1096-103. doi: 10.1139/m80-182.
Treatment of soybean root nodule tissue with a nonionic detergent. Nonidet P-40, after aldehyde fixation, results in a selective solubilization of membranes. The cell wall membrane of bacteroids and of free-living Rhizobium is resistant to this treatment. Fragments of "extra" membrane present inside the membrane envelope enclosing the bacteroids are also resistant to the detergent and are morphologically similar to the outer membrane of the cell wall of Rhizobium grown in broth culture. These observations, along with electrophoretic profiles of detergent resistant membranes from nodules, free-living Rhizobium, and isolated nodule bacteroids, suggest that the Rhizobium cell wall membrane undergoes significant changes during establishment of the root nodule symbiosis.
用非离子型去污剂Nonidet P - 40处理大豆根瘤组织，在醛固定后，会导致膜的选择性溶解。类菌体和自由生活的根瘤菌的细胞壁膜对这种处理具有抗性。包围类菌体的膜包膜内存在的“额外”膜碎片也对去污剂具有抗性，并且在形态上类似于在肉汤培养中生长的根瘤菌细胞壁的外膜。这些观察结果，连同来自根瘤、自由生活的根瘤菌和分离的根瘤类菌体的抗去污剂膜的电泳图谱，表明根瘤菌细胞壁膜在根瘤共生建立过程中发生了显著变化。
