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神经母细胞瘤细胞对电离辐射的反应：对人微血管内皮细胞体外侵袭和血管生成的调节

Response of neuroblastoma cells to ionizing radiation: modulation of in vitro invasiveness and angiogenesis of human microvascular endothelial cells.

作者信息

Jadhav Unmesh, Mohanam Sanjeeva

机构信息

Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine, Peoria, IL 61656, USA.

出版信息

Int J Oncol. 2006 Dec;29(6):1525-31.

PMID:17088992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2441915/
Abstract

Neuroblastomas are the most common extra-cranial tumors of childhood and well known for their heterogeneous clinical behavior associated with certain genetic aberrations. Radiation therapy is an important modality for the treatment of high-risk neuroblastomas. In this study, we investigated whether ionizing irradiation modulate the migration and invasiveness of human neuroblastoma cells and expression of proangiogenic molecules known to be involved in tumor progression and metastasis. Irradiation of neuroblastoma cells resulted in increased migration and invasion as measured by spheroid migration and matrigel invasion assay respectively. Zymographic analysis revealed an increase in enzyme activity of MMP-9 and uPA in conditioned medium of irradiated neuroblastoma cells compared with non-irradiated cells. An increase in VEGF levels was also found in lysates of irradiated neuroblastoma cells. The up-regulation of uPA, MMP-9 and VEGF transcripts was also confirmed by RT-PCR analysis. Next, we examined the irradiated tumor cell-mediated modulation of endothelial cell behavior. Conditioned media from irradiated neuroblastoma cells enhanced capillary-like structure formation of microvascular endothelial cells. In a coculture system, irradiation of neuroblastoma cells enhanced endothelial cell invasiveness through Matrigel matrix. Endothelial cells treated with irradiated tumor cell conditioned medium were also analyzed for expression of uPA, MMP-9 and VEGF and compared to cells treated with non-irradiated tumor cell conditioned medium. These findings suggest that the irradiation effects of tumor cells could influence endothelial angiogenesis present in non-irradiated fields.

摘要

神经母细胞瘤是儿童最常见的颅外肿瘤，以其与某些基因畸变相关的异质性临床行为而闻名。放射治疗是高危神经母细胞瘤治疗的重要方式。在本研究中，我们调查了电离辐射是否会调节人神经母细胞瘤细胞的迁移和侵袭能力以及已知参与肿瘤进展和转移的促血管生成分子的表达。分别通过球体迁移和基质胶侵袭试验测量发现，神经母细胞瘤细胞经照射后迁移和侵袭能力增强。酶谱分析显示，与未照射的细胞相比，照射后的神经母细胞瘤细胞条件培养基中MMP-9和尿激酶型纤溶酶原激活物（uPA）的酶活性增加。在照射后的神经母细胞瘤细胞裂解物中还发现血管内皮生长因子（VEGF）水平升高。RT-PCR分析也证实了uPA、MMP-9和VEGF转录本的上调。接下来，我们研究了照射后的肿瘤细胞对内皮细胞行为的调节作用。照射后的神经母细胞瘤细胞条件培养基增强了微血管内皮细胞毛细血管样结构的形成。在共培养系统中，神经母细胞瘤细胞经照射后增强了内皮细胞通过基质胶基质的侵袭能力。还分析了用照射后的肿瘤细胞条件培养基处理的内皮细胞中uPA、MMP-9和VEGF的表达，并与用未照射的肿瘤细胞条件培养基处理的细胞进行比较。这些发现表明，肿瘤细胞的照射效应可能会影响未照射区域的内皮血管生成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/20703467642b/nihms50747f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/e2eb8256e075/nihms50747f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/94fd204d1b25/nihms50747f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/b174046c24aa/nihms50747f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/20703467642b/nihms50747f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/e2eb8256e075/nihms50747f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/6ca038b559d6/nihms50747f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/4d4e3f19f3e7/nihms50747f3.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/94fd204d1b25/nihms50747f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/b174046c24aa/nihms50747f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f88/2441915/20703467642b/nihms50747f7.jpg

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2
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3
Angiogenesis and tumor growth inhibition by a matrix metalloproteinase inhibitor targeting radiation-induced invasion.一种靶向辐射诱导侵袭的基质金属蛋白酶抑制剂对血管生成和肿瘤生长的抑制作用
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J Neurooncol. 2006 Feb;76(3):227-37. doi: 10.1007/s11060-005-6499-4.
5
Tumor cell-associated neuropilin-1 and vascular endothelial growth factor expression as determinants of tumor growth in neuroblastoma.肿瘤细胞相关的神经纤毛蛋白-1和血管内皮生长因子表达作为神经母细胞瘤肿瘤生长的决定因素
Neuropathology. 2005 Sep;25(3):178-87. doi: 10.1111/j.1440-1789.2005.00610.x.
6
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