Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, University of Sadat City, Sadat city, Egypt.
Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Sci Rep. 2018 May 9;8(1):7423. doi: 10.1038/s41598-018-25586-3.
Ionizing radiation has cytotoxic and genotoxic effects caused mainly by the oxidative damage induced by free radical release. The need for radioprotectives is increasing to protect normal tissues during radiotherapy. In the present study, we investigated the radioprotective effect of Date syrup in rats subjected to whole body radiation at 6 Gy through biochemical, molecular and histopathological analysis. Significant elevations were recorded in the activities of serum ALT, AST, ALP and LDH and in the levels of all lipid profiles parameters, while the level of HDL-C was reduced. The concentration of liver MDA was elevated with depletion of hepatic glutathione (GSH) and catalase. DNA damage was evidenced by increased DNA strand breakage and DNA-protein crosslinks. Significant elevations were observed in the expression of liver TNF-α and serum activity of matrix metalloproteinase (MMP-9). Pretreatment of rats with Date syrup ameliorated the tissue damage induced by radiation as evidenced by the improvement of liver function, antioxidant status and reduction of DNA damage. Besides, liver TNF-α expression and serum MMP-9 activity were reduced. In conclusion, Date syrup could alleviate the toxic effects of ionizing radiation and thus is useful as a radioprotective in radiotherapy regimen.
电离辐射具有细胞毒性和遗传毒性作用，主要是由自由基释放引起的氧化损伤所致。为了在放射治疗过程中保护正常组织，对放射防护剂的需求正在增加。在本研究中，我们通过生化、分子和组织病理学分析，研究了枣糖浆在 6 Gy 全身照射大鼠中的放射防护作用。血清 ALT、AST、ALP 和 LDH 活性以及所有脂质谱参数水平显著升高，而高密度脂蛋白胆固醇（HDL-C）水平降低。肝 MDA 浓度升高，肝谷胱甘肽（GSH）和过氧化氢酶耗竭。DNA 损伤的证据是 DNA 链断裂和 DNA-蛋白质交联增加。肝 TNF-α和血清基质金属蛋白酶（MMP-9）活性的表达显著升高。枣糖浆预处理可改善辐射引起的组织损伤，表现为肝功能、抗氧化状态改善和 DNA 损伤减少。此外，肝 TNF-α表达和血清 MMP-9 活性降低。总之，枣糖浆可以减轻电离辐射的毒性作用，因此可作为放射治疗方案中的放射防护剂。
