Chen Ji-An, Li Xiang, Li Jun, Cao Jia, Qiu Zhiqun, Zhao Qing, Xu Chuan, Shu Weiqun
Department of Environmental Hygiene, College of Military Preventive Medicine, Third Military Medical University, Gaotanyan Street 30, Chongqing, 400038, China.
Appl Microbiol Biotechnol. 2007 Mar;74(3):676-82. doi: 10.1007/s00253-006-0700-3. Epub 2006 Nov 7.
In this study di-2-ethylhexyl phthalate (DEHP)-degradation strain CQ0110Y was isolated from activated sludge. According to the biophysical/biochemical characteristics and analysis of 16S rDNA, the strain was identified as Microbacterium sp. The results of this study showed the optimal pH value and optimal temperature which influenced the degradation rate in wastewater: pH 6.5-7.5, 25-35 degrees C. Kinetics of degradation reaction had been performed at different initial concentrations and different time. Analyzed with SPSS10.0 software, the DEHP degradation can be described as the same exponential model when the initial DEHP concentration was lower than 1,350 mg/l. The kinetics equation was ln C = -0.4087t + A, with the degradation half life of DEHP in wastewater (1.59 days). To the best of our knowledge, this is the first reported case of DEHP degradation by Microbacterium sp. strain.
在本研究中，从活性污泥中分离出邻苯二甲酸二（2-乙基己基）酯（DEHP）降解菌株CQ0110Y。根据生物物理/生化特性及16S rDNA分析，该菌株被鉴定为微杆菌属。本研究结果表明了影响废水中降解速率的最佳pH值和最佳温度：pH 6.5 - 7.5，25 - 35℃。在不同初始浓度和不同时间下进行了降解反应动力学研究。用SPSS10.0软件分析，当初始DEHP浓度低于1350 mg/l时，DEHP降解可用相同的指数模型描述。动力学方程为ln C = -0.4087t + A，DEHP在废水中的降解半衰期为1.59天。据我们所知，这是首次报道微杆菌属菌株降解DEHP的案例。
