Bastos Erick Leite, Ciscato Luiz Francisco Monteiro Leite, Bartoloni Fernando Heering, Catalani Luiz Henrique, Romoff Paulete, Baader Wilhelm Josef
Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-900 São Paulo, SP, Brazil.
Luminescence. 2007 Mar-Apr;22(2):126-33. doi: 10.1002/bio.935.
The use of poly(N-vinyl-2-pyrrolidone) (PVP) hydrogel-supported luminol chemiluminescence (CL) for the automatic determination of hydrogen peroxide and the quantification of the antiradical capacity of Trolox is described. The hydrogel containing luminol and hemin is prepared directly on a 96-well microplate and can be stored for up to 3 months without significant decrease in CL quantum yields. Furthermore, this system can also be used as a secondary light standard for the calibration of microplate luminometers.
描述了使用聚（N-乙烯基-2-吡咯烷酮）（PVP）水凝胶负载鲁米诺化学发光（CL）自动测定过氧化氢以及定量Trolox的抗自由基能力。含有鲁米诺和血红素的水凝胶直接在96孔微孔板上制备，可储存长达3个月，化学发光量子产率无显著下降。此外，该系统还可作为二级光标准用于微孔板发光计的校准。
