用于通过高效液相色谱-荧光检测法测定谷物和食品中赭曲霉毒素A的新型净化柱的性能

Performance of new clean-up column for the determination of ochratoxin A in cereals and foodstuffs by HPLC-FLD.

作者信息

Buttinger G, Fuchs E, Knapp H, Berthiller F, Schuhmacher R, Binder E-M, Krska R

机构信息

Konrad Lorenz Str. 20 A-3430 Tulln Austria.

出版信息

Food Addit Contam. 2004 Nov;21(11):1107-14. doi: 10.1080/02652030400023143.

DOI:10.1080/02652030400023143

PMID:15764340

Abstract

The performance of the newly developed Mycosep 229 Ochra and Multisep 229 Ochra clean-up columns for ochratoxin A (OTA) determination was evaluated. OTA was subsequently analysed using RP-HPLC with fluorescence detection. Recoveries for frequently contaminated commodities, like cereals, red wine, raisins and green coffee, were estimated. The recoveries obtained for the Mycosep 229 Ochra column were in the range from 87.9 +/- 12.5% (n = 6) for wheat to 99.4 +/- 2.7% (n = 24) for raisins. For Multisep 229 Ochra, recoveries from 76.5 +/- 8.0% (n = 6) for barley to 86.4 +/- 1.4% (n = 24) for raisins were achieved. Limits of detection for all matrices investigated (maize, wheat, rice, barley, raisins, green coffee beans, red wine) were in the range 0.4-2.4 microg kg(-1). The trueness of the method was tested using a certified reference material.

摘要

对新开发的用于测定赭曲霉毒素A（OTA）的Mycosep 229 Ochra和Multisep 229 Ochra净化柱的性能进行了评估。随后采用反相高效液相色谱法（RP-HPLC）结合荧光检测对OTA进行分析。对谷物、红酒、葡萄干和生咖啡豆等常见受污染商品的回收率进行了估算。Mycosep 229 Ochra柱的回收率范围为：小麦为87.9±12.5%（n = 6），葡萄干为99.4±2.7%（n = 24）。对于Multisep 229 Ochra柱，回收率范围为：大麦为76.5±8.0%（n = 6），葡萄干为86.4±1.4%（n = 24）。对所有研究基质（玉米、小麦、大米、大麦、葡萄干、生咖啡豆、红酒）的检测限在0.4 - 2.4 μg kg⁻¹范围内。使用有证标准物质对该方法的准确性进行了测试。