Toledo R, Espert A, Muñoz-Antoli C, Marcilla A, Fried B, Esteban J G
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain.
J Parasitol. 2005 Aug;91(4):978-80. doi: 10.1645/GE-461R.1.
The present study reports on the kinetics of antibodies and antigens in serum of mice experimentally infected with 75 metacercariae of Echinostoma caproni during the first 12 wk postinfection (wpi). Antibody titers in the serum of mice were determined by an indirect enzyme-linked immunosorbent assay (ELISA) using excretory/secretory (ES) antigens of E. caproni. The early detection of antibodies against ES antigens of E. caproni is feasible using indirect ELISA. Mice developed significant antibody responses at 2 wpi, and the values progressively increased until the end of the experiment. This may be related to the intestinal absorption of adult worm antigens that induces humoral responses. The presence of E. caproni circulating antigens was determined by a capture ELISA based on polyclonal rabbit antibodies against ES antigens of E. caproni. High levels of seroantigens in mice were detected by 1-2 wpi, probably because of the local inflammatory responses in mice induced by the adult worms. A drop in circulating antigen levels was observed at 9 wpi, which could reflect changes in the intestinal tissues over the course of the infection.
本研究报告了在感染卡氏棘口吸虫75个囊蚴后的前12周，实验感染小鼠血清中抗体和抗原的动力学变化。通过使用卡氏棘口吸虫排泄/分泌（ES）抗原的间接酶联免疫吸附测定（ELISA）来测定小鼠血清中的抗体滴度。使用间接ELISA可以早期检测出针对卡氏棘口吸虫ES抗原的抗体。小鼠在感染后2周出现显著的抗体反应，并且这些值在实验结束前逐渐增加。这可能与诱导体液反应的成虫抗原的肠道吸收有关。通过基于针对卡氏棘口吸虫ES抗原的多克隆兔抗体的捕获ELISA来测定卡氏棘口吸虫循环抗原的存在。在感染后1 - 2周检测到小鼠血清中高水平的抗原，这可能是由于成虫在小鼠体内诱导的局部炎症反应所致。在感染后9周观察到循环抗原水平下降，这可能反映了感染过程中肠道组织的变化。
