Klages Curtis
Air Force Research Laboratory (AFRL), Veterinary Resources, Veterinary Services Branch, AFRL, Brooks City Base, Texas, USA.
J Am Assoc Lab Anim Sci. 2006 Nov;45(6):49-53.
Automated plasmapheresis is an optimal method of plasma collection because the donor is a part of a closed loop where whole blood is withdrawn and separated and packed cells are returned in a serial fashion until the desired amount of plasma is obtained. The typical approach to collection of antibody-rich plasma involves withdrawal of whole blood from vaccinated animals, yielding approximately 1000 ml plasma from each animal, which is euthanized after this process. In the present study, 32 goats (Capra hircus) were vaccinated and conditioned for restraint in a modified Panepinto sling. Each animal was monitored clinically, including complete and differential blood counts and serum chemistries 24 h before and 24 to 48 h after each procedure. A jugular vein was surgically prepped, a 16-gauge needle catheter was placed, and the animal was attached to an automated plasmapheresis machine. After plasma removal, return of the resuspended packed blood cells, and infusion of 500 ml 0.9% NaCl, the animal was disconnected from the machine, the catheter removed, and the animal returned to the barn. There were no clinically significant changes in either the complete blood counts or the clinical chemistries during the course of this study. These 32 animals produced 240,000 ml of immunoglobulin-rich plasma over the course of this project and more than 949,000 ml of plasma to date. This study identifies a refinement in current antibody-recovery techniques and potentially reduces the number of animals necessary to produce bioreagents on a long-term and continual basis.
自动血浆置换术是一种理想的血浆采集方法，因为供血者是闭环系统的一部分，全血被抽出、分离，血细胞被依次回输，直至获得所需量的血浆。采集富含抗体血浆的典型方法是从接种疫苗的动物身上抽取全血，每只动物可产生约1000毫升血浆，采血后该动物会被安乐死。在本研究中，32只山羊（Capra hircus）接种疫苗并在改良的帕内平托吊床中进行约束适应。在每次操作前24小时以及操作后24至48小时，对每只动物进行临床监测，包括全血细胞计数、分类计数和血清生化检测。通过手术准备颈静脉，置入16号针头导管，然后将动物连接到自动血浆置换机上。去除血浆、回输重悬的血细胞并输注500毫升0.9%氯化钠溶液后，将动物与机器分离，拔出导管，动物返回畜舍。在本研究过程中，全血细胞计数和临床生化指标均未出现具有临床意义的变化。在本项目过程中，这32只动物产生了240,000毫升富含免疫球蛋白的血浆，迄今为止已产生超过949,000毫升血浆。本研究确定了当前抗体回收技术的一项改进方法，并有可能减少长期持续生产生物试剂所需的动物数量。
